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Literature summary extracted from

  • Vodnala, M.; Ranjbarian, F.; Pavlova, A.; de Koning, H.P.; Hofer, A.
    Trypanosoma brucei methylthioadenosine phosphorylase protects the parasite from the antitrypanosomal effect of deoxyadenosine: implications for the pharmacology of adenosine antimetabolites (2016), J. Biol. Chem., 291, 11717-11726.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.28 additional information the enzyme is not dependent on dithiothreitol Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.28 expressed in Escherichia coli BL21(DE3) pLysS cells Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.28 adenine
-
 Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.28 0.00009
-
 S-methyl-5'-thioadenosine at pH 7.4 and 37°C Trypanosoma brucei
2.4.2.28 0.005
-
 2'-fluoro-2'-deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei
2.4.2.28 0.0083
-
 deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei
2.4.2.28 0.013
-
 adenosine at pH 7.4 and 37°C Trypanosoma brucei
2.4.2.28 0.032
-
 2'-chloro-2'-deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.28 additional information the enzyme is not dependent on Na+, K+, or Mg2+ Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.28 34000
-
 x * 34000, SDS-PAGE Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.28 S-methyl-5'-thioadenosine + phosphate Trypanosoma brucei
-
 adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.28 S-methyl-5'-thioadenosine + phosphate Trypanosoma brucei TC221
-
 adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.28 Trypanosoma brucei
-
-
-
2.4.2.28 Trypanosoma brucei TC221
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.28 Ni-NTA column chromatography and Sephadex G-25 gel filtration Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.28 2'-chloro-2'-deoxyadenosine + phosphate
-
 Trypanosoma brucei adenine + 2-chloro-2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.28 2'-chloro-2'-deoxyadenosine + phosphate
-
 Trypanosoma brucei TC221 adenine + 2-chloro-2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.28 2'-fluoro-2'-deoxyadenosine + phosphate
-
 Trypanosoma brucei adenine + 2'-fluoro-2'-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.28 2'-fluoro-2'-deoxyadenosine + phosphate
-
 Trypanosoma brucei TC221 adenine + 2'-fluoro-2'-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.28 adenosine + phosphate
-
 Trypanosoma brucei adenine + D-ribose 1-phosphate
-
 ?
2.4.2.28 adenosine + phosphate
-
 Trypanosoma brucei TC221 adenine + D-ribose 1-phosphate
-
 ?
2.4.2.28 deoxyadenosine + phosphate
-
 Trypanosoma brucei adenine + deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.28 additional information very low activity with cordycepin, Ara-A, tubercidin, guanosine, deoxyguanosine, inosine, deoxyinosine, uridine, deoxyuridine, cytidine, deoxycytidine, and thymidine Trypanosoma brucei ?
-
 ?
2.4.2.28 additional information very low activity with cordycepin, Ara-A, tubercidin, guanosine, deoxyguanosine, inosine, deoxyinosine, uridine, deoxyuridine, cytidine, deoxycytidine, and thymidine Trypanosoma brucei TC221 ?
-
 ?
2.4.2.28 S-methyl-5'-thioadenosine + phosphate
-
 Trypanosoma brucei adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.28 S-methyl-5'-thioadenosine + phosphate
-
 Trypanosoma brucei TC221 adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.28 ? x * 34000, SDS-PAGE Trypanosoma brucei

Synonyms

EC Number Synonyms Comment Organism
2.4.2.28 methylthioadenosine phosphorylase
-
 Trypanosoma brucei
2.4.2.28 MTAP
-
 Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.28 2.13
-
 S-methyl-5'-thioadenosine at pH 7.4 and 37°C Trypanosoma brucei
2.4.2.28 5.4
-
 2'-fluoro-2'-deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei
2.4.2.28 6.4
-
 2'-chloro-2'-deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei
2.4.2.28 8.4
-
 adenosine at pH 7.4 and 37°C Trypanosoma brucei
2.4.2.28 9.6
-
 deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.28 5.5 8 the enzyme activity is stable between pH 5.5 and 8.0 Trypanosoma brucei

General Information

EC Number General Information Comment Organism
2.4.2.28 malfunction there is increased deoxyadenosine sensitivity in Trypanosoma brucei methylthioadenosine phosphorylase knockdown cells Trypanosoma brucei
2.4.2.28 physiological function the enzyme protects Trypanosoma brucei from the antitrypanosomal effect of deoxyadenosine Trypanosoma brucei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.28 200
-
 2'-chloro-2'-deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei
2.4.2.28 650
-
 adenosine at pH 7.4 and 37°C Trypanosoma brucei
2.4.2.28 1080
-
 2'-fluoro-2'-deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei
2.4.2.28 1160
-
 deoxyadenosine at pH 7.4 and 37°C Trypanosoma brucei
2.4.2.28 24000
-
 S-methyl-5'-thioadenosine at pH 7.4 and 37°C Trypanosoma brucei