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Literature summary extracted from

  • Lyons, B.; Ravulapalli, R.; Lanoue, J.; Lugo, M.R.; Dutta, D.; Carlin, S.; Merrill, A.R.
    Scabin, a Novel DNA-acting ADP-ribosyltransferase from Streptomyces scabies (2016), J. Biol. Chem., 291, 11198-11215.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.30 expressed in Escherichia coli BL21(DE3) cells Streptomyces scabiei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.30 in complex with inhibitor PJ34 or P6, hanging drop vapor diffusion method, using 0.1 M MES, pH 6.5, and 15% (w/v) PEG 400 Streptomyces scabiei

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.30 Q158A/E160A the mutant shows reduced activity compared to the wild type enzyme Streptomyces scabiei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.30 2-[[3-(dimethylamino)-2-oxopropyl]amino]-5,6-dihydrophenanthridin-6-one potent inhibitor Streptomyces scabiei
2.4.2.30 4-[8-fluoro-6-oxo-1H,2H,3H,4H,5H,6H-benzo[c]1,6-naphthyridin-2-yl]butanoic acid
-
 Streptomyces scabiei
2.4.2.30 8-fluoro-2-[3-(piperidin-1-yl)propanesulfonyl]-1H,2H,3H,4H,5H,6H-benzo[c]1,6-naphthyridin-6-one
-
 Streptomyces scabiei
2.4.2.30 8-fluoro-2-[3-(piperidin-1-yl)propyl]-1H,2H,3H,4H,5H,6H-benzo[c]1,6-naphthyridin-6-one
-
 Streptomyces scabiei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.30 0.068
-
 NAD+ wild type enzyme, at pH 7.9 and 22°C Streptomyces scabiei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.30 21962
-
 x * 21962, MALDI-TOF mass spectrometry Streptomyces scabiei
2.4.2.30 22000
-
 x * 22000, SDS-PAGE Streptomyces scabiei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-acceptor Streptomyces scabiei the enzyme labels the exocyclic amino group on guanine bases in either single-stranded or double-stranded DNA nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+
-
 ?
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-acceptor Streptomyces scabiei 87.22 the enzyme labels the exocyclic amino group on guanine bases in either single-stranded or double-stranded DNA nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.30 Streptomyces scabiei C9Z6T8
-
-
2.4.2.30 Streptomyces scabiei 87.22 C9Z6T8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.30 HiTrap chelating Sepharose column chromatography and HiTrap Q-Sepharose column chromatography Streptomyces scabiei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.30 additional information the enzyme also targets deoxyguanosine Streptomyces scabiei ?
-
 ?
2.4.2.30 additional information the enzyme also targets deoxyguanosine Streptomyces scabiei 87.22 ?
-
 ?
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-acceptor the enzyme labels the exocyclic amino group on guanine bases in either single-stranded or double-stranded DNA Streptomyces scabiei nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+
-
 ?
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-acceptor the enzyme labels the exocyclic amino group on guanine bases in either single-stranded or double-stranded DNA Streptomyces scabiei 87.22 nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.30 ? x * 22000, SDS-PAGE Streptomyces scabiei
2.4.2.30 ? x * 21962, MALDI-TOF mass spectrometry Streptomyces scabiei

Synonyms

EC Number Synonyms Comment Organism
2.4.2.30 ADP-ribosyltransferase
-
 Streptomyces scabiei
2.4.2.30 scabin
-
 Streptomyces scabiei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.30 0.005
-
 NAD+ mutant enzyme Q158A/E160A, at pH 7.9 and 22°C Streptomyces scabiei
2.4.2.30 1.57
-
 NAD+ wild type enzyme, at pH 7.9 and 22°C Streptomyces scabiei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.30 0.003
-
 2-[[3-(dimethylamino)-2-oxopropyl]amino]-5,6-dihydrophenanthridin-6-one wild type enzyme, at pH 7.9 and 22°C Streptomyces scabiei
2.4.2.30 0.007
-
 8-fluoro-2-[3-(piperidin-1-yl)propanesulfonyl]-1H,2H,3H,4H,5H,6H-benzo[c]1,6-naphthyridin-6-one wild type enzyme, at pH 7.9 and 22°C Streptomyces scabiei
2.4.2.30 0.018
-
 4-[8-fluoro-6-oxo-1H,2H,3H,4H,5H,6H-benzo[c]1,6-naphthyridin-2-yl]butanoic acid wild type enzyme, at pH 7.9 and 22°C Streptomyces scabiei
2.4.2.30 0.019
-
 8-fluoro-2-[3-(piperidin-1-yl)propyl]-1H,2H,3H,4H,5H,6H-benzo[c]1,6-naphthyridin-6-one wild type enzyme, at pH 7.9 and 22°C Streptomyces scabiei
2.4.2.30 0.024
-
 4-[8-fluoro-6-oxo-1H,2H,3H,4H,5H,6H-benzo[c]1,6-naphthyridin-2-yl]butanoic acid wild type enzyme, at pH 7.9 and 22°C Streptomyces scabiei