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Literature summary extracted from

  • Brammer Basta, L.A.; Ghosh, A.; Pan, Y.; Jakoncic, J.; Lloyd, E.P.; Townsend, C.A.; Lamichhane, G.; Bianchet, M.A.
    Loss of a functionally and structurally distinct LD-transpeptidase, LdtMt5, compromises cell wall integrity in Mycobacterium tuberculosis (2015), J. Biol. Chem., 290, 25670-25685.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.12 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.2.12 hanging drop vapor diffusion method, using 85 mM sodium citrate, pH 5.6, 25.5% (w/v) polyethylene glycol 4000, 170 mM ammonium acetate, and 15% (v/v) glycerol Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.12 doripenem
-
 Mycobacterium tuberculosis
2.3.2.12 ertapenem
-
 Mycobacterium tuberculosis
2.3.2.12 Imipenem
-
 Mycobacterium tuberculosis
2.3.2.12 meropenem
-
 Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.12 peptidyl-tRNA1 + aminoacyl-tRNA2 Mycobacterium tuberculosis
-
 tRNA1 + peptidyl(aminoacyl-tRNA2)
-
 ?
2.3.2.12 peptidyl-tRNA1 + aminoacyl-tRNA2 Mycobacterium tuberculosis CDC 1551
-
 tRNA1 + peptidyl(aminoacyl-tRNA2)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.12 Mycobacterium tuberculosis P9WKV2
-
-
2.3.2.12 Mycobacterium tuberculosis CDC 1551 P9WKV2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.12 Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.12 additional information the enzyme shows hydrolytic activity with nitrocefin (Km of 0.097 mM, kcat of 0.013 s-1, and kcat/Km of 145 1/sec*mM, at pH 10.0) Mycobacterium tuberculosis ?
-
 ?
2.3.2.12 additional information the enzyme shows hydrolytic activity with nitrocefin (Km of 0.097 mM, kcat of 0.013 s-1, and kcat/Km of 145 1/sec*mM, at pH 10.0) Mycobacterium tuberculosis CDC 1551 ?
-
 ?
2.3.2.12 peptidyl-tRNA1 + aminoacyl-tRNA2
-
 Mycobacterium tuberculosis tRNA1 + peptidyl(aminoacyl-tRNA2)
-
 ?
2.3.2.12 peptidyl-tRNA1 + aminoacyl-tRNA2
-
 Mycobacterium tuberculosis CDC 1551 tRNA1 + peptidyl(aminoacyl-tRNA2)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.12 LD-transpeptidase
-
 Mycobacterium tuberculosis
2.3.2.12 LdtMt5
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.3.2.12 malfunction Mycobacterium tuberculosis lacking a functional copy of LdtMt5 displays aberrant growth and is more susceptible to killing by crystal violet, osmotic shock, and select carbapenem antibiotics Mycobacterium tuberculosis
2.3.2.12 physiological function the enzyme LdtMt5 is necessary for properly maintaining cell wall integrity Mycobacterium tuberculosis