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Literature summary extracted from

  • Borisova, A.S.; Isaksen, T.; Dimarogona, M.; Kognole; A.A.; Mathiesen, G.; Varnai, A.; Rohr, A.K.; Payne, C.M.; Sorlie, M.; Sandgren, M.; Eijsink, V.G.
    Structural and functional characterization of a lytic polysaccharide monooxygenase with broad substrate specificity (2015), J. Biol. Chem., 290, 22955-22969.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.B10
-
 Neurospora crassa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.99.B10 crystals grown in presence of Zn2+ and of Cu2+. Structure of the catalytic domain reveals an extended, highly polar substrate-binding surface well suited to interact with a variety of sugar substrates. Binding affinities are in the low micromolar range for polymeric substrates due in part to the presence of a carbohydrate-binding module Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.B10 Cu2+ the Cu+ center environment is altered upon substrate binding. The oxidative regioselectivity of LPMO9 enzymes (C1, C4, or both) correlates with distinct structural features of the copper coordination sphere. In strictly C1-oxidizing LPMO9s, access to the solvent-facing axial coordination position is restricted by a conserved tyrosine residue, whereas access to this same position seems unrestricted in C4-oxidizing LPMO9s Neurospora crassa
1.14.99.54 Cu2+ The copper ion lies in the center of a flat surface that interacts with the substrate. The equatorial plane includes the protein's N-terminal Ndelta of His-1 and the Nepsilon of His-83 Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.B10 Neurospora crassa Q7SHI8
-
-
1.14.99.54 Neurospora crassa Q7SHI8
-
-
1.14.99.54 Neurospora crassa DSM 1257 Q7SHI8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.B10 beta-glucan + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O
-
 ?
1.14.99.B10 beta-glucan + ascorbate + O2
-
 Neurospora crassa DSM 1257 ? + dehydroascorbate + H2O
-
 ?
1.14.99.B10 cello-oligosaccharides + ascorbate + O2 with a degree of polymerization of 5 and 7 Neurospora crassa ? + dehydroascorbate + H2O
-
 ?
1.14.99.B10 cello-oligosaccharides + ascorbate + O2 with a degree of polymerization of 5 and 7 Neurospora crassa DSM 1257 ? + dehydroascorbate + H2O
-
 ?
1.14.99.B10 low molecular lignin + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O
-
 ?
1.14.99.B10 additional information enzyme acts both on cellulose and on noncellulose beta-glucans, including cellodextrins and xyloglucan Neurospora crassa ?
-
 ?
1.14.99.B10 additional information enzyme acts both on cellulose and on noncellulose beta-glucans, including cellodextrins and xyloglucan Neurospora crassa DSM 1257 ?
-
 ?
1.14.99.B10 thio-linked cello-oligosaccharides + ascorbate + O2 with a degree of polymerization of 2–5 Neurospora crassa ? + dehydroascorbate + H2O
-
 ?
1.14.99.B10 thio-linked xylopentaose + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O
-
 ?
1.14.99.B10 xylogluco-oligosaccharides + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O
-
 ?
1.14.99.54 xyloglucan + ascorbate + O2
-
 Neurospora crassa ?
-
 ?
1.14.99.54 xyloglucan + ascorbate + O2
-
 Neurospora crassa DSM 1257 ?
-
 ?
1.14.99.54 [(1->4)-beta-D-xylosyl]6-(1->4)-beta-D-glucose + ascorbate + O2
-
 Neurospora crassa ?
-
 ?
1.14.99.54 [(1->4)-beta-D-xylosyl]6-(1->4)-beta-D-glucose + ascorbate + O2
-
 Neurospora crassa DSM 1257 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.B10 LPMO9C
-
 Neurospora crassa