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Literature summary extracted from

  • Cahoon, R.E.; Lutke, W.K.; Cameron, J.C.; Chen, S.; Lee, S.G.; Rivard, R.S.; Rea, P.A.; Jez, J.M.
    Adaptive engineering of phytochelatin-based heavy metal tolerance (2015), J. Biol. Chem., 290, 17321-17330.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.15 expressed in Escherichia coli BL21(DE3) cells and Saccharomyces cerevisiae strain TY167 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.15 A182G/A282V/G329S the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 A59V the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 C109Y the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 C91S/A199S the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 D71N the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 E52K the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 F83C/N170D the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 Q48R/C144Y/G168S/W280R the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 R74H/S230C/L250R the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 S51T/N143I/N170I/H220R the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 S60C/S202I the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 T123R/F163I the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 T139P the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 V181G the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 V97C the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 V97L the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana
2.3.2.15 Y186C the mutant has strongly reduced activity compared to the wild type enzyme. The mutant improves the Cd2+ tolerance of Arabidopsis thaliana Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.15 55000
-
 x * 55000, FLAG-tagged enzyme, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly Arabidopsis thaliana
-
 Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.15 Arabidopsis thaliana Q9S7Z3
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.15 1.3
-
 mutant enzyme Y186C, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 1.8
-
 mutant enzyme C109Y, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 2.6
-
 mutant enzyme T139P, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 2.8
-
 mutant enzyme V181G, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 2.8
-
 mutant enzyme V97C, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 2.9
-
 mutant enzyme A59V, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 3.1
-
 mutant enzyme F83C/N170D, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 3.2
-
 mutant enzyme E52K, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 3.3
-
 mutant enzyme S51T/N143I/N170I/H220R, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 3.6
-
 mutant enzyme T123R/F163I, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 3.7
-
 mutant enzyme R74H/S230C/L250R, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 4.6
-
 mutant enzyme C91S/A199S, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 4.8
-
 mutant enzyme V97L, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 5
-
 mutant enzyme Q48R/C144Y/G168S/W280R, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 5.1
-
 mutant enzyme D71N, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 5.5
-
 mutant enzyme A182G/A282V/G329S, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 5.9
-
 mutant enzyme S60C/S202I, at pH 8.0 and 30°C Arabidopsis thaliana
2.3.2.15 16.8
-
 wild type enzyme, at pH 8.0 and 30°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.15 glutathione + [Glu(-Cys)]n-Gly
-
 Arabidopsis thaliana Gly + [Glu(-Cys)]n+1-Gly
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.15 ? x * 55000, FLAG-tagged enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.3.2.15 PCS1
-
 Arabidopsis thaliana
2.3.2.15 phytochelatin synthase
-
 Arabidopsis thaliana