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Literature summary extracted from

  • Imamura, K.; Maeda, S.; Kawamura, I.; Matsuyama, K.; Shinohara, N.; Yahiro, Y.; Nagano, S.; Setoguchi, T.; Yokouchi, M.; Ishidou, Y.; Komiya, S.
    Human immunodeficiency virus type 1 enhancer-binding protein 3 is essential for the expression of asparagine-linked glycosylation 2 in the regulation of osteoblast and chondrocyte differentiation (2014), J. Biol. Chem., 289, 9865-9879.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.132 gene ALG2, expression analysis by quantitative RT-PCR, coexpression of Alg2 and Runx2 in COS-7 cells, both localizing to different compartments Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.132 additional information ALG2 downregulation in ST-2 cells by siRNA transfection Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.132 endoplasmic reticulum
-
 Mus musculus 5783
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Organism

EC Number Organism UniProt Comment Textmining
2.4.1.132 Mus musculus Q9DBE8 gene ALG2
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2.4.1.132 Mus musculus C57/BL6J Q9DBE8 gene ALG2
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.132 chondrocyte
-
 Mus musculus
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2.4.1.132 MC3T3-E1 cell a calvarial bone-derived osteoblast cell line Mus musculus
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2.4.1.132 additional information asparagine-linked glycosylation 2 (Alg2) is commonly downregulated in BMP-2-induced osteoblast differentiation in both MC3T3-E1 and ST-2 cells Mus musculus
-
2.4.1.132 osteoblast
-
 Mus musculus
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2.4.1.132 ST-2 cell
-
 Mus musculus
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.132 asparagine-linked glycosylation 2
-
 Mus musculus

Expression

EC Number Organism Comment Expression
2.4.1.132 Mus musculus knockdown of human immunodeficiency virus type 1 enhancer-binding protein 3, Hivep3, downregulates Alg2 expression. Expression of the Alg2 gene is reduced upon knockdown of Hivep3 in osteoblastic cells down

General Information

EC Number General Information Comment Organism
2.4.1.132 malfunction loss of Alg2 promotes osteoblast differentiation in ST-2 cells without affecting the protein level of Runx2. Alg2 knockdown does not affect endoplasmic reticulum stress or bone morphogenetic protein, BMP, signaling in ST-2 cells. Atf4,also known as CCAAT/enhancer-binding protein homologous protein (Chop), is mildly upregulated by sAlg2, but only in BMP-2-treated cells. The expression of a target of the Atf6 pathway, heat shock protein 5 (Hspa5), is not altered by loss of Alg2 Mus musculus
2.4.1.132 metabolism asparagine-linked glycosylation (ALG) is one of the most common protein modification reactions in eukaryotic cells, as many proteins that are translocated across or integrated into the rough endoplasmic reticulum carry N-linked oligosaccharides. Linkage between an asparagine-linked glycosylation mannosyltransferase gene and osteochondrogenesis Mus musculus
2.4.1.132 physiological function human immunodeficiency virus type 1 enhancer-binding protein 3, Hivep3 or Schnurri-3, Zas3, and Krc, is essential for the expression of asparagine-linked glycosylation 2 in the regulation of osteoblast and chondrocyte differentiation. Alg2 suppresses osteoblast differentiation by inhibiting the activity of Runx2. Alg2 silencing suppresses the expression of Creb3l2 and chondrogenesis. Enzyme ALG2 is associated with osteochondrogenesis, Alg2 is a downstream mediator of Hivep3 and suppresses osteogenesis, whereas it promotes chondrogenesis. Regulation analysis, overview Mus musculus