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Literature summary extracted from

  • Sinha, A.; Koehrer, C.; Weber, M.H.; Masuda, I.; Mootha, V.K.; Hou, Y.M.; RajBhandary, U.L.
    Biochemical characterization of pathogenic mutations in human mitochondrial methionyl-tRNA formyltransferase (2014), J. Biol. Chem., 289, 32729-32741.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.9 A172L site-directed mutagenesis, the mutation causes a 6fold decrease in enzyme activity compared to wild-type, the Vmax/Km of the mutant is about 4fold lower than that of the wild-type enzyme Escherichia coli
2.1.2.9 A172S site-directed mutagenesis, the mutation does not significantly affect the enzyme activity Escherichia coli
2.1.2.9 A89L site-directed mutagenesis, the Vmax/Km of the mutant is about 144fold lower than that of the wild-type enzyme Escherichia coli
2.1.2.9 A89S site-directed mutagenesis, the mutation does not significantly affect the enzyme activity Escherichia coli
2.1.2.9 S125L naturally occurring mutation, the mutant has a 653fold lower activity compared to the wild-type Homo sapiens
2.1.2.9 S209L naturally occurring mutation, the mutant has a 36fold lower activity compared to the wild-type Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.9 additional information
-
 additional information Michaelis-Menten kinetics of wild-type and mutant enzymes Escherichia coli
2.1.2.9 additional information
-
 additional information Michaelis-Menten kinetics of wild-type and mutant enzymes with human mitochondrial mt-tRNAMet and with Escherichia coli tRNA2fMet, overview Homo sapiens
2.1.2.9 0.00051
-
 L-methionyl-tRNAfMet Escherichia coli tRNA2fMet, pH 7.6, 37°C, wild-type enzyme Escherichia coli
2.1.2.9 0.00107
-
 L-methionyl-tRNAfMet Escherichia coli tRNA2fMet, pH 7.6, 37°C, mutant A172L Escherichia coli
2.1.2.9 0.00587
-
 L-methionyl-tRNAfMet Escherichia coli tRNA2fMet, pH 7.6, 37°C, mutant A89L Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.9 mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.2.9 Mg2+ required Escherichia coli
2.1.2.9 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet Escherichia coli
-
 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet Homo sapiens
-
 tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.9 Escherichia coli P23882
-
-
2.1.2.9 Homo sapiens Q96DP5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet
-
 Homo sapiens tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet Escherichia coli tRNA2fMet Escherichia coli tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?
2.1.2.9 10-formyltetrahydrofolate + L-methionyl-tRNAfMet human mt-tRNAMet Homo sapiens tetrahydrofolate + N-formylmethionyl-tRNAfMet
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.2.9 mitochondrial methionyl-tRNA formyltransferase
-
 Escherichia coli
2.1.2.9 mitochondrial methionyl-tRNA formyltransferase
-
 Homo sapiens
2.1.2.9 MTF
-
 Escherichia coli
2.1.2.9 MTF
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.9 37
-
 assay at Escherichia coli
2.1.2.9 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.9 7.6
-
 assay at Escherichia coli
2.1.2.9 7.6
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.9 10-formyltetrahydrofolate
-
 Escherichia coli
2.1.2.9 10-formyltetrahydrofolate
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.1.2.9 malfunction mutations of conserved enzyme residues affect enzyme activity and lead to poor formylation of mitochondrial methionyl-tRNA and thereby reduced mitochondrial translation efficiency, causing Leigh syndrome Escherichia coli
2.1.2.9 malfunction mutations of conserved enzyme residues affect enzyme activity and lead to poor formylation of mitochondrial methionyl-tRNA and thereby reduced mitochondrial translation efficiency, causing Leigh syndrome Homo sapiens
2.1.2.9 physiological function N-formylation of initiator methionyl-tRNA (Met-tRNAMet) by methionyl-tRNA formyltransferase is important for translation initiation in bacteria, mitochondria, and chloroplasts Escherichia coli
2.1.2.9 physiological function N-formylation of initiator methionyl-tRNA (Met-tRNAMet) by methionyl-tRNA formyltransferase is important for translation initiation in bacteria, mitochondria, and chloroplasts Homo sapiens