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Literature summary extracted from

  • Zhang, Z.; Wu, J.; Lin, W.; Wang, J.; Yan, H.; Zhao, W.; Ma, J.; Ding, J.; Zhang, P.; Zhao, G.P.
    Subdomain II of alpha-isopropylmalate synthase is essential for activity: inferring a mechanism of feedback inhibition (2014), J. Biol. Chem., 289, 27966-27978.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.13 expressed in Escherichia coli BL21(DE3) cells Leptospira biflexa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.3.13 hanging drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5), 0.8 mM sodium formate, and 30% (w/v) PEG 2000 monomethyl ether Leptospira biflexa

General Stability

EC Number General Stability Organism
2.3.3.13 partial deletion of the regulatory domain of isoform IPMS1 results in a loss of about 50% of the catalytic activity Leptospira biflexa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.13 L-leucine
-
Leptospira biflexa
2.3.3.13 Zn2+ Zn2+ slightly inhibits the enzyme activity Leptospira biflexa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.13 K+ an excess amount of 50 mM K+ is included in the standard enzymatic assays. Only isoform IPMS1 is coactivated by monovalent metal ions like K+ Leptospira biflexa
2.3.3.13 Mn2+ most effective activator, an excess amount 2 mM Mn2+ is included in the standard enzymatic assays Leptospira biflexa
2.3.3.13 NH4+ only isoform IPMS1 is coactivated by monovalent metal ions like NH4+ Leptospira biflexa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Leptospira biflexa
-
(2S)-2-isopropylmalate + CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.13 Leptospira biflexa B0SN40
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.13 Ni-NTA column chromatography Leptospira biflexa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Leptospira biflexa (2S)-2-isopropylmalate + CoA
-
?
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutyrate + H2O
-
Leptospira biflexa ?
-
?
2.3.3.13 acetyl-CoA + 3-methyl-2-oxoisovalerate + H2O
-
Leptospira biflexa ?
-
?
2.3.3.13 acetyl-CoA + 3-methyl-2-oxovalerate + H2O
-
Leptospira biflexa ?
-
?
2.3.3.13 additional information isoform IPMS2 shows no activity with 4-methylthio-2-oxobutyrate or 2-oxohexanoate Leptospira biflexa ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.13 Alpha-isopropylmalate synthase
-
Leptospira biflexa
2.3.3.13 IPMS1 long enzyme form Leptospira biflexa
2.3.3.13 IPMS2 short enzyme form Leptospira biflexa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.13 37
-
isoform IPMS1 Leptospira biflexa
2.3.3.13 50
-
isoform IPMS2 Leptospira biflexa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.13 45
-
the stability of isoform IPMS2 isremarkably decreased when the temperature exceeds 45°C and is higher than that of isoform IPMS1 at 37°C Leptospira biflexa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.13 8.5
-
-
Leptospira biflexa