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Literature summary extracted from

  • Wang, J.; Nemeria, N.S.; Chandrasekhar, K.; Kumaran, S.; Arjunan, P.; Reynolds, S.; Calero, G.; Brukh, R.; Kakalis, L.; Furey, W.; Jordan, F.
    Structure and function of the catalytic domain of the dihydrolipoyl acetyltransferase component in Escherichia coli pyruvate dehydrogenase complex (2014), J. Biol. Chem., 289, 15215-15230.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.12 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.12 His9-tagged catalytic domain, hanging drop vapor diffusion method, using 0.2 M potassium thiocyanate and 20% (w/v) polyethylene glycol 3350 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.12 H602C inactive Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.12 30028
-
 x * 30028, catalytic domain, calculated from amino acid sequence Escherichia coli
2.3.1.12 30030
-
 x * 30030, catalytic domain, FT mass spectrometry Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.12 acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine Escherichia coli
-
 CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.12 Escherichia coli P06959
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.12 nickel affinity resin column chromatography, Superdex G75 gel filtration, and G3000SW TSK gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.12 acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine
-
 Escherichia coli CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine
-
 r
2.3.1.12 CoA + enzyme N6-(S-acetyldihydrolipoyl)lysine
-
 Escherichia coli acetyl-CoA + enzyme N6-(dihydrolipoyl)lysine
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.12 ? x * 30028, catalytic domain, calculated from amino acid sequence Escherichia coli
2.3.1.12 ? x * 30030, catalytic domain, FT mass spectrometry Escherichia coli
2.3.1.12 trimer x-ray crystallography Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.3.1.12 dihydrolipoamide acetyltransferase
-
 Escherichia coli
2.3.1.12 pyruvate dehydrogenase complex component E2p
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.12 thiamine diphosphate
-
 Escherichia coli