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Literature summary extracted from

  • Soysa, R.; Wilson, Z.N.; Elferich, J.; Forquer, I.; Shinde, U.; Riscoe, M.K.; Yates, P.A.; Ullman, B.
    Substrate inhibition of uracil phosphoribosyltransferase by uracil can account for the uracil growth sensitivity of Leishmania donovani pyrimidine auxotrophs (2013), J. Biol. Chem., 288, 29954-29964.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.9 expressed in Escherichia coli BL21Star (DE3) One Shot cells Leishmania donovani

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.9 4-thiouracil substrate inhibition at high concentrations Leishmania donovani
2.4.2.9 5-fluorouracil substrate inhibition at high concentrations Leishmania donovani
2.4.2.9 EDTA
-
 Leishmania donovani
2.4.2.9 Uracil substrate inhibition at high concentrations Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.9 0.0064
-
 5-fluorouracil at pH 7.5 and 25°C Leishmania donovani
2.4.2.9 0.0071
-
 4-thiouracil at pH 7.5 and 25°C Leishmania donovani
2.4.2.9 0.0204
-
 Uracil at pH 7.5 and 25°C Leishmania donovani
2.4.2.9 0.0993
-
 5-phospho-alpha-D-ribose 1-diphosphate with uracil as cosubstrate, at pH 7.5 and 25°C Leishmania donovani

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.9 Co2+ can replace Mg2+ Leishmania donovani
2.4.2.9 Mg2+ maximum activity in the presence of 5 mM Mg2+ Leishmania donovani
2.4.2.9 Mn2+ can replace Mg2+ Leishmania donovani
2.4.2.9 additional information not stimulated by Ba2+, Ca2+, Ni2+, and Zn2+ Leishmania donovani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.9 27000
-
 4 * 27000, SDS-PAGE Leishmania donovani
2.4.2.9 100000
-
 gel filtration Leishmania donovani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate Leishmania donovani
-
 UMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Leishmania donovani
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.9 Ni-NTA column chromatography Leishmania donovani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 4-thiouracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Leishmania donovani 4-thio-UMP + diphosphate
-
 ?
2.4.2.9 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Leishmania donovani 5-fluoro-UMP + diphosphate
-
 ?
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Leishmania donovani UMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.9 homotetramer 4 * 27000, SDS-PAGE Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
2.4.2.9 UPRT
-
 Leishmania donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.9 8
-
-
 Leishmania donovani

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.9 7.5 8.5 more than 90% activity between 7.5 and 8.5 with sharp drops in activity 0.5 pH units outside this range Leishmania donovani