BRENDA - Enzyme Database show

Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissimum L.) seed catalyzing the selective production of trilinolenin

Pan, X.; Siloto, R.M.; Wickramarathna, A.D.; Mietkiewska, E.; Weselake, R.J.; J. Biol. Chem. 288, 24173-24188 (2013)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.158
alpha-linolenic acid
stimulation; stimulation
Linum usitatissimum
Cloned(Commentary)
EC Number
Commentary
Organism
2.3.1.158
expression in Saccharomyces cerevisiae; expression in Saccharomyces cerevisiae
Linum usitatissimum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.158
76890
-
x * 76890, calculated
Linum usitatissimum
2.3.1.158
79072
-
x * 79072, calculated
Linum usitatissimum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.158
Linum usitatissimum
V5LWK6
-
-
2.3.1.158
Linum usitatissimum
V5LY10
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.158
additional information
low expression in seed
Linum usitatissimum
-
2.3.1.158
seed
preferential expression
Linum usitatissimum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.158
linolenoyl-phosphatidylcholine + 1,2-dilinolenoylglycerol
-
736422
Linum usitatissimum
lyso-phosphatidylcholine + trilinolenoylglycerol
-
-
-
?
2.3.1.158
additional information
in the presence of alpha-linolenic acid, isoform PDAT1 produces triacylglycerol with a specific fatty acid composition. PDAT1 has the ability to synthesize trilinolenin, which is the major molecular species of triacylglycerol in flax oil
736422
Linum usitatissimum
?
-
-
-
-
2.3.1.158
additional information
in the presence of alpha-linolenic acid, isoform PDAT2 produces triacylglycerol with a specific fatty acid composition. PDAT2 has the ability to synthesize trilinolenin, which is the major molecular species of triacylglycerol in flax oil
736422
Linum usitatissimum
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.3.1.158
?
x * 76890, calculated; x * 79072, calculated
Linum usitatissimum
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.3.1.158
Linum usitatissimum
calculated
-
6.4
2.3.1.158
Linum usitatissimum
calculated
-
8.2
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.158
alpha-linolenic acid
stimulation
Linum usitatissimum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.158
expression in Saccharomyces cerevisiae
Linum usitatissimum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.158
76890
-
x * 76890, calculated
Linum usitatissimum
2.3.1.158
79072
-
x * 79072, calculated
Linum usitatissimum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.158
additional information
low expression in seed
Linum usitatissimum
-
2.3.1.158
seed
preferential expression
Linum usitatissimum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.158
linolenoyl-phosphatidylcholine + 1,2-dilinolenoylglycerol
-
736422
Linum usitatissimum
lyso-phosphatidylcholine + trilinolenoylglycerol
-
-
-
?
2.3.1.158
additional information
in the presence of alpha-linolenic acid, isoform PDAT1 produces triacylglycerol with a specific fatty acid composition. PDAT1 has the ability to synthesize trilinolenin, which is the major molecular species of triacylglycerol in flax oil
736422
Linum usitatissimum
?
-
-
-
-
2.3.1.158
additional information
in the presence of alpha-linolenic acid, isoform PDAT2 produces triacylglycerol with a specific fatty acid composition. PDAT2 has the ability to synthesize trilinolenin, which is the major molecular species of triacylglycerol in flax oil
736422
Linum usitatissimum
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.3.1.158
?
x * 76890, calculated
Linum usitatissimum
2.3.1.158
?
x * 79072, calculated
Linum usitatissimum
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.3.1.158
Linum usitatissimum
calculated
-
6.4
2.3.1.158
Linum usitatissimum
calculated
-
8.2
General Information
EC Number
General Information
Commentary
Organism
2.3.1.158
physiological function
expression of isoform PDAT1 restores triacylglycerol synthesis in Saccharomyces cerevisiae H1246 when culturing yeast in the presence of alpha-linolenic acid; expression of isoform PDAT1 restores triacylglycerol synthesis in Saccharomyces cerevisiae H1246 when culturing yeast in the presence of alpha-linolenic acid
Linum usitatissimum
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.3.1.158
physiological function
expression of isoform PDAT1 restores triacylglycerol synthesis in Saccharomyces cerevisiae H1246 when culturing yeast in the presence of alpha-linolenic acid
Linum usitatissimum