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Literature summary extracted from

  • Weng, Q.; Elder, J.; Jacobson, G.
    Site-specific mutagenesis of residues in the Escherichia coli mannitol permease that have been suggested to be important for its phosphorylation and chemoreception functions (1992), J. Biol. Chem., 267, 19529-19535.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.197 expressed in Escherichia coli LGS322 cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.197 C384D inactive Escherichia coli
2.7.1.197 C384H inactive Escherichia coli
2.7.1.197 H156A the mutant shows wild type activity Escherichia coli
2.7.1.197 H195A the mutant shows very low activity compared to the wild type enzyme Escherichia coli
2.7.1.197 H195N the mutant shows 30% of wild type activity Escherichia coli
2.7.1.197 H195R the mutant shows very low activity compared to the wild type enzyme Escherichia coli
2.7.1.197 H554A inactive Escherichia coli
2.7.1.197 H554D inactive Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.197 membrane
-
 Escherichia coli 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.197 65000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.197 [protein]-Npi-phospho-L-histidine + D-mannitol[side 1] Escherichia coli
-
 [protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.197 Escherichia coli P00550
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.197 [protein]-Npi-phospho-L-histidine + D-mannitol[side 1]
-
 Escherichia coli [protein]-L-histidine + D-mannitol 1-phosphate[side 2]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.197 EIIMtl
-
 Escherichia coli
2.7.1.197 mannitol permease
-
 Escherichia coli
2.7.1.197 mtlA
-
 Escherichia coli