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Literature summary extracted from

  • Jacobson, G.; Saraceni-Richards, C.
    The Escherichia coli mannitol permease as a model for transport via the bacterial phosphotransferase system (1993), J. Bioenerg. Biomembr., 25, 621-626.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.197 E218A the mutant has a high Km for mannitol but can carry out facilitated diffusion of mannitol at a rate higher than the wild type protein in its unphosphorylated form Escherichia coli
2.7.1.197 E257A the mutant does not detectably bind mannitol up to a concentration of at least 1 mM Escherichia coli
2.7.1.197 G253E the mutant cannot transport but still can phosphorylate mannitol Escherichia coli
2.7.1.197 H195A the mutant has a 40fold higher KD for mannitol compared to the wild type protein and is defective in mannitol phosphorylation even at high mannitol concentrations Escherichia coli
2.7.1.197 H195R the mutant has a 40fold higher KD for mannitol compared to the wild type protein and is defective in mannitol phosphorylation even at high mannitol concentrations Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.197 membrane
-
Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.197 [protein HPr]-Npi-phospho-L-histidine + D-mannitol[side 1] Escherichia coli
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[protein HPr]-L-histidine + D-mannitol 1-phosphate[side 2]
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.197 Escherichia coli P00550
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.197 [protein HPr]-Npi-phospho-L-histidine + D-mannitol[side 1]
-
Escherichia coli [protein HPr]-L-histidine + D-mannitol 1-phosphate[side 2]
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.197 mannitol permease the enzyme consists of two cytoplasmic domains (EIIA and EIIB) involved in mannitol phosphorylation and an integral membrane domain (EIIC) which is sufficient to bind mannitol, but which transports mannitol at a rate that is dependent on phosphorylation of the EIIA and EIIB domains Escherichia coli