EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.197 | E218A | the mutant has a high Km for mannitol but can carry out facilitated diffusion of mannitol at a rate higher than the wild type protein in its unphosphorylated form | Escherichia coli |
2.7.1.197 | E257A | the mutant does not detectably bind mannitol up to a concentration of at least 1 mM | Escherichia coli |
2.7.1.197 | G253E | the mutant cannot transport but still can phosphorylate mannitol | Escherichia coli |
2.7.1.197 | H195A | the mutant has a 40fold higher KD for mannitol compared to the wild type protein and is defective in mannitol phosphorylation even at high mannitol concentrations | Escherichia coli |
2.7.1.197 | H195R | the mutant has a 40fold higher KD for mannitol compared to the wild type protein and is defective in mannitol phosphorylation even at high mannitol concentrations | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.197 | membrane | - |
Escherichia coli | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.197 | [protein HPr]-Npi-phospho-L-histidine + D-mannitol[side 1] | Escherichia coli | - |
[protein HPr]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.197 | Escherichia coli | P00550 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.197 | [protein HPr]-Npi-phospho-L-histidine + D-mannitol[side 1] | - |
Escherichia coli | [protein HPr]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.197 | mannitol permease | the enzyme consists of two cytoplasmic domains (EIIA and EIIB) involved in mannitol phosphorylation and an integral membrane domain (EIIC) which is sufficient to bind mannitol, but which transports mannitol at a rate that is dependent on phosphorylation of the EIIA and EIIB domains | Escherichia coli |