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Literature summary extracted from

  • Eram, M.S.; Wong, A.; Oduaran, E.; Ma, K.
    Molecular and biochemical characterization of bifunctional pyruvate decarboxylases and pyruvate ferredoxin oxidoreductases from Thermotoga maritima and Thermotoga hypogea (2015), J. Biochem., 158, 459-466.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.1 0.021
-
 CoA pH 8.4, 80°C Pseudothermotoga hypogea
1.2.7.1 0.063
-
 CoA pH 8.4, 80°C Thermotoga maritima
1.2.7.1 0.13
-
 pyruvate pH 8.4, 80°C Pseudothermotoga hypogea
1.2.7.1 0.14
-
 oxidized methyl viologen pH 8.4, 80°C Pseudothermotoga hypogea
1.2.7.1 0.4
-
 pyruvate pH 8.4, 80°C Thermotoga maritima
4.1.1.1 0.92
-
 pyruvate pH 8.4, 80°C Thermotoga maritima
4.1.1.1 1.4
-
 pyruvate pH 8.4, 80°C Pseudothermotoga hypogea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.1 13000
-
 1 * 46000 + 1 * 35000 + 1 * 23000 + 1 * 13000, SDS-PAGE Thermotoga maritima
1.2.7.1 14000
-
 1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE Pseudothermotoga hypogea
1.2.7.1 22000
-
 1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE Pseudothermotoga hypogea
1.2.7.1 23000
-
 1 * 46000 + 1 * 35000 + 1 * 23000 + 1 * 13000, SDS-PAGE Thermotoga maritima
1.2.7.1 35000
-
 1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE Pseudothermotoga hypogea
1.2.7.1 35000
-
 1 * 46000 + 1 * 35000 + 1 * 23000 + 1 * 13000, SDS-PAGE Thermotoga maritima
1.2.7.1 45000
-
 1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE Pseudothermotoga hypogea
1.2.7.1 46000
-
 1 * 46000 + 1 * 35000 + 1 * 23000 + 1 * 13000, SDS-PAGE Thermotoga maritima
1.2.7.1 156000
-
 gel filtration Thermotoga maritima
1.2.7.1 157000
-
 gel filtration Pseudothermotoga hypogea
4.1.1.1 13000
-
 1 * 46000 plus 1 * 35000 plus 1 * 23000 plus 1 * 13000, SDS-PAGE Thermotoga maritima
4.1.1.1 14000
-
 1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE Pseudothermotoga hypogea
4.1.1.1 22000
-
 1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE Pseudothermotoga hypogea
4.1.1.1 23000
-
 1 * 46000 plus 1 * 35000 plus 1 * 23000 plus 1 * 13000, SDS-PAGE Thermotoga maritima
4.1.1.1 35000
-
 1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE Pseudothermotoga hypogea
4.1.1.1 35000
-
 1 * 46000 plus 1 * 35000 plus 1 * 23000 plus 1 * 13000, SDS-PAGE Thermotoga maritima
4.1.1.1 45000
-
 1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE Pseudothermotoga hypogea
4.1.1.1 46000
-
 1 * 46000 plus 1 * 35000 plus 1 * 23000 plus 1 * 13000, SDS-PAGE Thermotoga maritima
4.1.1.1 156000
-
 gel filtration Thermotoga maritima
4.1.1.1 157000
-
 gel filtration Pseudothermotoga hypogea

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.1 Pseudothermotoga hypogea V9NGI4 and V9NFX1 and V9NGQ2 and V9NHA1 V9NGI4, i.e. subunit PorB, V9NFX1, i.e. subunit PorD, V9NGQ2 i.e. subunit PorG, V9NHA1 i.e. subunit PorA, bifunctional pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase
-
1.2.7.1 Thermotoga maritima Q56317 and 05650 and Q56316 and O05651 Q56317, i.e. subunit PorB, O05650, i.e. subunit PorC, Q56316 i.e. subunit PorD, O05651 i.e. subunit PorA, bifunctional pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase
-
1.2.7.1 Thermotoga maritima DSM 3109 Q56317 and 05650 and Q56316 and O05651 Q56317, i.e. subunit PorB, O05650, i.e. subunit PorC, Q56316 i.e. subunit PorD, O05651 i.e. subunit PorA, bifunctional pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase
-
4.1.1.1 Pseudothermotoga hypogea V9NGI4 and V9NFX1 and V9NGQ2 and V9NHA1 V9NGI4, i.e. subunit PorB, V9NFX1, i.e. subunit PorD, V9NGQ2 i.e. subunit PorG, V9NHA1 i.e. subunit PorA, bifunctional pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase
-
4.1.1.1 Thermotoga maritima Q56317 and 05650 and Q56316 and O05651 Q56317, i.e. subunit PorB, O05650, i.e. subunit PorC, Q56316 i.e. subunit PorD, O05651 i.e. subunit PorA, bifunctional pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase
-
4.1.1.1 Thermotoga maritima DSM 3109 Q56317 and 05650 and Q56316 and O05651 Q56317, i.e. subunit PorB, O05650, i.e. subunit PorC, Q56316 i.e. subunit PorD, O05651 i.e. subunit PorA, bifunctional pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.1 both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity are extremely oxygen sensitive, 50% loss of activity upon exposure to air at room temperature within 15 min Thermotoga maritima
1.2.7.1 both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity are extremely oxygen sensitive, 50% loss of activity upon exposure to air at room temperature within 15 min Pseudothermotoga hypogea
4.1.1.1 both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity are extremely oxygen sensitive, 50% loss of activity upon exposure to air at room temperature within 15 min Thermotoga maritima
4.1.1.1 both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity are extremely oxygen sensitive, 50% loss of activity upon exposure to air at room temperature within 15 min Pseudothermotoga hypogea

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.1 purified enzyme shos a brownish colour characteristic of iron-sulfur containing proteins Thermotoga maritima
1.2.7.1 purified enzyme shows a brownish colour characteristic of iron-sulfur containing proteins Pseudothermotoga hypogea
4.1.1.1 purified enzyme shos a brownish colour characteristic of iron-sulfur containing proteins Thermotoga maritima
4.1.1.1 purified enzyme shows a brownish colour characteristic of iron-sulfur containing proteins Pseudothermotoga hypogea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.7.1 78
-
 pH 8.4, 80°C Thermotoga maritima
1.2.7.1 119
-
 pH 8.4, 80°C Pseudothermotoga hypogea
4.1.1.1 1.4
-
 pH 8.4, 80°C Thermotoga maritima
4.1.1.1 1.9
-
 pH 8.4, 80°C Pseudothermotoga hypogea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 pyruvate + CoA + 2 oxidized methyl viologen
-
 Thermotoga maritima acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + 2 oxidized methyl viologen
-
 Pseudothermotoga hypogea acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+
-
 ?
1.2.7.1 pyruvate + CoA + 2 oxidized methyl viologen
-
 Thermotoga maritima DSM 3109 acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+
-
 ?
4.1.1.1 pyruvate
-
 Thermotoga maritima acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate
-
 Pseudothermotoga hypogea acetaldehyde + CO2
-
 ?
4.1.1.1 pyruvate
-
 Thermotoga maritima DSM 3109 acetaldehyde + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.1 tetramer 1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE Pseudothermotoga hypogea
1.2.7.1 tetramer 1 * 46000 + 1 * 35000 + 1 * 23000 + 1 * 13000, SDS-PAGE Thermotoga maritima
4.1.1.1 tetramer 1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE Pseudothermotoga hypogea
4.1.1.1 tetramer 1 * 46000 plus 1 * 35000 plus 1 * 23000 plus 1 * 13000, SDS-PAGE Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.1 80
-
 pyruvate decarboxylase activity Pseudothermotoga hypogea
1.2.7.1 90
-
-
 Thermotoga maritima
1.2.7.1 90
-
 pyruvate ferredoxin oxidoreductase activity Pseudothermotoga hypogea
4.1.1.1 80
-
 pyruvate decarboxylase activity Pseudothermotoga hypogea
4.1.1.1 90
-
 pyruvate ferredoxin oxidoreductase activity Pseudothermotoga hypogea
4.1.1.1 95
-
 above 95°C, activity is highly temperature dependent Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.7.1 80
-
 half-life of pyruvate decarboxylase activity 2 h, of pyruvate ferredoxin oxidoreductase activity 3 h Pseudothermotoga hypogea
4.1.1.1 80
-
 half-life of pyruvate decarboxylase activity 2 h, of pyruvate ferredoxin oxidoreductase activity 3 h Pseudothermotoga hypogea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.1 8.4
-
 both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity Thermotoga maritima
1.2.7.1 8.4
-
 both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity Pseudothermotoga hypogea
4.1.1.1 8.4
-
 both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity Thermotoga maritima
4.1.1.1 8.4
-
 both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity Pseudothermotoga hypogea

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.1 CoA pyruvate ferredoxin oxidoreductase activity is strictly CoA-dependent, desulfo-CoA does not serve as cofactor Thermotoga maritima
1.2.7.1 CoA pyruvate ferredoxin oxidoreductase activity is strictly CoA-dependent, desulfo-CoA does not serve as cofactor. The pyruvate decarboxylase activity accepts desulfo-CoA Pseudothermotoga hypogea
4.1.1.1 CoA Km value 0.0031 mM Thermotoga maritima
4.1.1.1 CoA pyruvate ferredoxin oxidoreductase activity is strictly CoA-dependent, desulfo-CoA does not serve as cofactor. The pyruvate decarboxylase activity accepts desulfo-CoA, Km value for CoA is 0.0014 mM Pseudothermotoga hypogea