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Literature summary extracted from

  • Kim, M.; Le, H.; McInerney, M.J.; Buckel, W.
    Identification and characterization of re-citrate synthase in Syntrophus aciditrophicus (2013), J. Bacteriol., 195, 1689-1696.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.3 expressed in Escherichia coli BL21 CodonPlus (DE3)-GroEL cells Syntrophus aciditrophicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.3 2-oxoglutarate 40% inhibition at 10 mM Syntrophus aciditrophicus
2.3.3.3 4-hydroxymercuribenzoate complete inhibition at 0.1 mM Syntrophus aciditrophicus
2.3.3.3 5,5'-dithiobis-2-nitrobenzoate 30% inhibition at 10 mM Syntrophus aciditrophicus
2.3.3.3 ATP 30% inhibition at 10 mM Syntrophus aciditrophicus
2.3.3.3 citrate 40% inhibition at 10 mM Syntrophus aciditrophicus
2.3.3.3 EDTA complete inhibition at 0.2 mM Syntrophus aciditrophicus
2.3.3.3 additional information not inhibited by 5 mM NADH Syntrophus aciditrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.3 0.085
-
 oxaloacetate at pH 8.0 and 22°C Syntrophus aciditrophicus
2.3.3.3 0.13
-
 acetyl-CoA at pH 8.0 and 22°C Syntrophus aciditrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.3 Ca2+ the enzyme contains stoichiometric amounts of Ca2+ (0.9 Ca/73 kDa) Syntrophus aciditrophicus
2.3.3.3 Co2+ Co2+ increases the activity by about 70% compared with that obtained withMn2+ Syntrophus aciditrophicus
2.3.3.3 Mg2+ Mg2+ has no effect at all on enzyme activity Syntrophus aciditrophicus
2.3.3.3 Mn2+ assays are performed in the presence of 0.2 mM MnCl2 Syntrophus aciditrophicus
2.3.3.3 Zn2+ the EDTA-inactivated enzyme regains activity by addition Zn2+ at final concentrations of 0.2 mM Syntrophus aciditrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.3 65000
-
 4 * 65000, SDS-PAGE Syntrophus aciditrophicus
2.3.3.3 71800
-
 4 * 71800, calculated from amino acid sequence Syntrophus aciditrophicus
2.3.3.3 72891
-
 4 * 72891, electrospray-time of flight mass spectrometry Syntrophus aciditrophicus
2.3.3.3 271000
-
 gel filtration Syntrophus aciditrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.3 acetyl-CoA + H2O + oxaloacetate Syntrophus aciditrophicus specific substrates (R)-citrate + CoA
-
 ?
2.3.3.3 acetyl-CoA + H2O + oxaloacetate Syntrophus aciditrophicus ATCC 700169 specific substrates (R)-citrate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.1 no activity in Syntrophus aciditrophicus
-
-
-
2.3.3.3 Syntrophus aciditrophicus
-
-
-
2.3.3.3 Syntrophus aciditrophicus ATCC 700169
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.3 Strep-tactin column chromatography and Superdex 200 gel filtration Syntrophus aciditrophicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.3 culture condition:crotonate-grown cell
-
 Syntrophus aciditrophicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.3 0.72
-
 purified enzyme, in the absence of Mn2+, at pH 8.0 and 22°C Syntrophus aciditrophicus
2.3.3.3 1.2
-
 purified enzyme, in the presence of 0.2 mM Mn2+, at pH 8.0 and 22°C Syntrophus aciditrophicus

Storage Stability

EC Number Storage Stability Organism
2.3.3.3 -80°C, the oxically purified enzyme, 20% loss of activity after each cycle of freezing at -80°C and thawing Syntrophus aciditrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.3 acetyl-CoA + H2O + oxaloacetate specific substrates Syntrophus aciditrophicus (R)-citrate + CoA
-
 ?
2.3.3.3 acetyl-CoA + H2O + oxaloacetate specific substrates Syntrophus aciditrophicus ATCC 700169 (R)-citrate + CoA
-
 ?
2.3.3.3 additional information no activity with propionyl-CoA, 2-oxoglutarate, pyruvate, and 2-oxo-3-methylbutanoate Syntrophus aciditrophicus ?
-
 ?
2.3.3.3 additional information no activity with propionyl-CoA, 2-oxoglutarate, pyruvate, and 2-oxo-3-methylbutanoate Syntrophus aciditrophicus ATCC 700169 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.3 homotetramer 4 * 65000, SDS-PAGE Syntrophus aciditrophicus
2.3.3.3 homotetramer 4 * 71800, calculated from amino acid sequence Syntrophus aciditrophicus
2.3.3.3 homotetramer 4 * 72891, electrospray-time of flight mass spectrometry Syntrophus aciditrophicus

Synonyms

EC Number Synonyms Comment Organism
2.3.3.3 Re-citrate synthase
-
 Syntrophus aciditrophicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.3 -80
-
 the oxically purified enzyme loses 20% of its activity after each cycle of freezing at -80°C and thawing Syntrophus aciditrophicus