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Literature summary extracted from

  • Dauvillee, D.; Kinderf, I.S.; Li, Z.; Kosar-Hashemi, B.; Samuel, M.S.; Rampling, L.; Ball, S.; Morell, M.K.
    Role of the Escherichia coli glgX gene in glycogen metabolism (2005), J. Bacteriol., 187, 1465-1473.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.196 expressed in Escherichia coli BL21AI cells Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.196 76000
-
 x * 76000, His6-tagged enzyme, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.196 Escherichia coli
-
-
-
3.2.1.196 Escherichia coli BW25113
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.196 Ni-NTA agarose column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.196 additional information the isoamylase-type debranching enzyme shows high specificity for hydrolysis of chains consisting of three or four glucose residues Escherichia coli ?
-
 ?
3.2.1.196 additional information the isoamylase-type debranching enzyme shows high specificity for hydrolysis of chains consisting of three or four glucose residues Escherichia coli BW25113 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.196 ? x * 76000, His6-tagged enzyme, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.2.1.196 GlgX
-
 Escherichia coli
3.2.1.196 isoamylase-type debranching enzyme
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
3.2.1.196 metabolism the enzyme is predominantly involved in glycogen catabolism by selectively debranching the polysaccharide outer chains that were previously recessed by glycogen phosphorylase Escherichia coli