EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.196 | expressed in Escherichia coli BL21AI cells | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.196 | 76000 | - |
x * 76000, His6-tagged enzyme, SDS-PAGE | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.196 | Escherichia coli | - |
- |
- |
3.2.1.196 | Escherichia coli BW25113 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.196 | Ni-NTA agarose column chromatography | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.196 | additional information | the isoamylase-type debranching enzyme shows high specificity for hydrolysis of chains consisting of three or four glucose residues | Escherichia coli | ? | - |
? | |
3.2.1.196 | additional information | the isoamylase-type debranching enzyme shows high specificity for hydrolysis of chains consisting of three or four glucose residues | Escherichia coli BW25113 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.196 | ? | x * 76000, His6-tagged enzyme, SDS-PAGE | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.196 | GlgX | - |
Escherichia coli |
3.2.1.196 | isoamylase-type debranching enzyme | - |
Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.196 | metabolism | the enzyme is predominantly involved in glycogen catabolism by selectively debranching the polysaccharide outer chains that were previously recessed by glycogen phosphorylase | Escherichia coli |