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Literature summary extracted from

  • Bayly, R.C.; Chapman, P.J.; Dagley, S.; Di Berardino, D.
    Purification and some properties of maleylpyruvate hydrolase and fumarylpyruvate hydrolase from Pseudomonas alcaligenes (1980), J. Bacteriol., 143, 70-77.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7.1.23 0.014
-
 3-maleylpyruvate pH and temperature not specified in the publication Pseudomonas alcaligenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.7.1.23 Mn2+ activates Pseudomonas alcaligenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.7.1.23 33000
-
 2 * 33000, SDS-PAGE Pseudomonas alcaligenes
3.7.1.23 77000
-
 gel filtration Pseudomonas alcaligenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.7.1.23 3-maleylpyruvate + H2O Pseudomonas alcaligenes 100% activity maleate + pyruvate
-
 ?
3.7.1.23 3-maleylpyruvate + H2O Pseudomonas alcaligenes NCIB 9867 100% activity maleate + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.7.1.23 Pseudomonas alcaligenes
-
-
-
3.7.1.23 Pseudomonas alcaligenes NCIB 9867
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.7.1.23 ammonium sulfate precipitation, DEAE-cellulose column chromatography, Sephadex G-150 gel filtration, DEAE-Sephadex A50 gel filtration, and triethylanilnoethyl-cellulose column chromatography Pseudomonas alcaligenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.7.1.23 0.17
-
 crude extract, pH and temperature not specified in the publication Pseudomonas alcaligenes
3.7.1.23 8.8
-
 after 52fold purification, pH and temperature not specified in the publication Pseudomonas alcaligenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.7.1.23 3-maleylpyruvate + H2O 100% activity Pseudomonas alcaligenes maleate + pyruvate
-
 ?
3.7.1.23 3-maleylpyruvate + H2O 100% activity Pseudomonas alcaligenes NCIB 9867 maleate + pyruvate
-
 ?
3.7.1.23 6-bromomaleylpyruvate + H2O 130% activity compared to 3-maleylpyruvate Pseudomonas alcaligenes 2-bromomaleate + pyruvate
-
 ?
3.7.1.23 6-bromomaleylpyruvate + H2O 130% activity compared to 3-maleylpyruvate Pseudomonas alcaligenes NCIB 9867 2-bromomaleate + pyruvate
-
 ?
3.7.1.23 6-chloromaleylpyruvate + H2O 130% activity compared to 3-maleylpyruvate Pseudomonas alcaligenes 2-chloromaleate + pyruvate
-
 ?
3.7.1.23 6-chloromaleylpyruvate + H2O 130% activity compared to 3-maleylpyruvate Pseudomonas alcaligenes NCIB 9867 2-chloromaleate + pyruvate
-
 ?
3.7.1.23 6-ethylmaleylpyruvate + H2O 60% activity compared to 3-maleylpyruvate Pseudomonas alcaligenes 2-ethylmaleate + pyruvate
-
 ?
3.7.1.23 6-ethylmaleylpyruvate + H2O 60% activity compared to 3-maleylpyruvate Pseudomonas alcaligenes NCIB 9867 2-ethylmaleate + pyruvate
-
 ?
3.7.1.23 6-fluoromaleylpyruvate + H2O 100% activity compared to 3-maleylpyruvate Pseudomonas alcaligenes 2-fluoromaleate + pyruvate
-
 ?
3.7.1.23 6-methylmaleylpyruvate + H2O 110% activity compared to 3-maleylpyruvate Pseudomonas alcaligenes 2-methylmaleate + pyruvate
-
 ?
3.7.1.23 6-propylmaleylpyruvate + H2O 10% activity compared to 3-maleylpyruvate Pseudomonas alcaligenes 2-propylmaleate + pyruvate
-
 ?
3.7.1.23 additional information no activity against either fumarylpyruvate, maleylacetoacetate, or fumarylacetoacetate Pseudomonas alcaligenes ?
-
 ?
3.7.1.23 additional information no activity against either fumarylpyruvate, maleylacetoacetate, or fumarylacetoacetate Pseudomonas alcaligenes NCIB 9867 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.7.1.23 homodimer 2 * 33000, SDS-PAGE Pseudomonas alcaligenes

Synonyms

EC Number Synonyms Comment Organism
3.7.1.23 MPH11
-
 Pseudomonas alcaligenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7.1.23 7.2 7.7
-
 Pseudomonas alcaligenes