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Literature summary extracted from

  • Huang, H.; Carter, M.S.; Vetting, M.W.; Al-Obaidi, N.; Patskovsky, Y.; Almo, S.C.; Gerlt, J.A.
    A general strategy for the discovery of metabolic pathways: D-threitol, L-threitol, and erythritol utilization in Mycobacterium smegmatis (2015), J. Am. Chem. Soc., 137, 14570-14573.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.209 0.003
-
 L-erythrulose pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.209 0.0106
-
 D-erythrulose pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.209 0.0378
-
 dihydroxyacetone pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.210 0.0046
-
 D-erythrulose pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.210 0.339
-
 dihydroxyacetone pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.210 0.396
-
 D-erythrulose pH and temperature not specified in the publication Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.403 D-threitol + NAD+ Mycolicibacterium smegmatis
-
 D-erythrulose + NADH + H+
-
 ?
2.7.1.209 ATP + L-erythrulose Mycolicibacterium smegmatis the enzyme participates in the degradation of L-threitol ADP + L-erythrulose 4-phosphate
-
 ?
2.7.1.210 ATP + D-erythrulose Mycolicibacterium smegmatis the enzyme participates in the degradation of erythritol and D-threitol ADP + D-erythrulose 4-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.403 Mycolicibacterium smegmatis A0QYC2
-
-
2.7.1.209 Mycolicibacterium smegmatis A0R758
-
-
2.7.1.210 Mycolicibacterium smegmatis A0QXE4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.403 D-threitol + NAD+
-
 Mycolicibacterium smegmatis D-erythrulose + NADH + H+
-
 ?
2.7.1.209 ATP + D-erythrulose catalytic efficiency is 4fold lower compared to L-erythrulose as substrate Mycolicibacterium smegmatis ADP + D-erythrulose 4-phosphate
-
 ?
2.7.1.209 ATP + dihydroxyacetone catalytic efficiency is about 100fold lower compared to L-erythrulose as substrate Mycolicibacterium smegmatis ?
-
 ?
2.7.1.209 ATP + L-erythrulose
-
 Mycolicibacterium smegmatis ADP + L-erythrulose 4-phosphate
-
 ?
2.7.1.209 ATP + L-erythrulose the enzyme participates in the degradation of L-threitol Mycolicibacterium smegmatis ADP + L-erythrulose 4-phosphate
-
 ?
2.7.1.210 ATP + D-erythrulose
-
 Mycolicibacterium smegmatis ADP + D-erythrulose 4-phosphate
-
 ?
2.7.1.210 ATP + D-erythrulose the enzyme participates in the degradation of erythritol and D-threitol Mycolicibacterium smegmatis ADP + D-erythrulose 4-phosphate
-
 ?
2.7.1.210 ATP + dihydroxyacetone catalytic efficiency is about 3500fold lower compared to D-erythrulose Mycolicibacterium smegmatis ADP + ?
-
 ?
2.7.1.210 ATP + L-erythrulose catalytic efficiency is about 3000fold lower compared to D-erythrulose Mycolicibacterium smegmatis ADP + L-erythrulose 4-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.403 D-threitol dehydrogenase
-
 Mycolicibacterium smegmatis
1.1.1.403 dthD
-
 Mycolicibacterium smegmatis
2.7.1.209 lerK
-
 Mycolicibacterium smegmatis
2.7.1.210 derK
-
 Mycolicibacterium smegmatis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.209 1.6
-
 D-erythrulose pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.209 1.79
-
 L-erythrulose pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.209 2.12
-
 dihydroxyacetone pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.210 0.063
-
 dihydroxyacetone pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.210 0.091
-
 D-erythrulose pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.210 2.88
-
 D-erythrulose pH and temperature not specified in the publication Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.403 NAD+
-
 Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
2.7.1.209 metabolism the enzyme participates in the degradation of L-threitol Mycolicibacterium smegmatis
2.7.1.210 metabolism the enzyme participates in the degradation of erythritol and D-threitol Mycolicibacterium smegmatis
5.1.3.39 metabolism the enzyme participates in the degradation of L-threitol Mycolicibacterium smegmatis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.209 56.1
-
 dihydroxyacetone pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.209 150.9
-
 D-erythrulose pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.209 597
-
 L-erythrulose pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.210 0.186
-
 dihydroxyacetone pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.210 0.23
-
 D-erythrulose pH and temperature not specified in the publication Mycolicibacterium smegmatis
2.7.1.210 626
-
 D-erythrulose pH and temperature not specified in the publication Mycolicibacterium smegmatis