BRENDA - Enzyme Database

Pseudoglycosyltransferase catalyzes nonglycosidic C-N coupling in validamycin a biosynthesis

Asamizu, S.; Yang, J.; Almabruk, K.H.; Mahmud, T.; J. Am. Chem. Soc. 133, 12124-12135 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.5.1.135
expressed in Escherichia coli BL21(DE3) cells
Streptomyces hygroscopicus subsp. limoneus
3.1.3.101
expression in Escherichia coli
Streptomyces hygroscopicus subsp. limoneus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.135
0.0597
-
GDP-valienol
at pH 7.5 and 30°C
Streptomyces hygroscopicus subsp. limoneus
2.5.1.135
0.308
-
validamine 7-phosphate
at pH 7.5 and 30°C
Streptomyces hygroscopicus subsp. limoneus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.91
Mg2+
in reactions containing 1 mM Mg2+, the enzyme effectively catalyzes the conversion of valienol 1-phosphate to GDP-valienol and less effectively to ADP-valienol or other NDP derivatives. In reactions containing 10 mM Mg2+ the enzyme more effectively uses ATP as nucleotidyl donor than GTP or other NTPs
Streptomyces hygroscopicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.5.1.135
GDP-valienol + validamine 7-phosphate
Streptomyces hygroscopicus subsp. limoneus
-
validoxylamine A 7'-phosphate + GDP
-
-
?
3.1.3.101
validoxylamine A 7'-phosphate + H2O
Streptomyces hygroscopicus subsp. limoneus
the enhzyme is involved in the biosynthesis of the antifungal agent validamycin A
validoxylamine A + phosphate
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.5.1.135
Streptomyces hygroscopicus subsp. limoneus
Q15JG1
-
-
2.7.7.91
Streptomyces hygroscopicus
-
-
-
2.7.7.91
Streptomyces hygroscopicus 5008
-
-
-
3.1.3.101
Streptomyces hygroscopicus subsp. limoneus
Q15JF8
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.5.1.135
Ni-NTA resin column chromatography
Streptomyces hygroscopicus subsp. limoneus
3.1.3.101
-
Streptomyces hygroscopicus subsp. limoneus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.5.1.135
GDP-valienol + validamine 7-phosphate
-
736302
Streptomyces hygroscopicus subsp. limoneus
validoxylamine A 7'-phosphate + GDP
-
-
-
?
2.5.1.135
additional information
incubation of the enzyme with UDP- or GDP-glucose and glucose 6-phosphate does not give any product. No activity with validoxylamine A and UDP-glucose
736302
Streptomyces hygroscopicus subsp. limoneus
?
-
-
-
?
2.7.7.91
ATP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus
diphosphate + ADP-valienol
-
-
-
?
2.7.7.91
ATP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus 5008
diphosphate + ADP-valienol
-
-
-
?
2.7.7.91
CTP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus
diphosphate + CDP-valienol
-
-
-
?
2.7.7.91
CTP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus 5008
diphosphate + CDP-valienol
-
-
-
?
2.7.7.91
dTTP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus
diphosphate + dTDP-valienol
-
-
-
?
2.7.7.91
dTTP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus 5008
diphosphate + dTDP-valienol
-
-
-
?
2.7.7.91
GTP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus
diphosphate + GDP-valienol
-
-
-
?
2.7.7.91
GTP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus 5008
diphosphate + GDP-valienol
-
-
-
?
2.7.7.91
additional information
in reactions containing 1 mM Mg2+, the enzyme effectively catalyzes the conversion of valienol 1-phosphate to GDP-valienol and less effectively to ADP-valienol or other NDP derivatives. In reactions containing 10 mM Mg2+ the enzyme more effectively uses ATP as nucleotidyl donor than GTP or other NTPs
736302
Streptomyces hygroscopicus
?
-
-
-
?
2.7.7.91
additional information
in reactions containing 1 mM Mg2+, the enzyme effectively catalyzes the conversion of valienol 1-phosphate to GDP-valienol and less effectively to ADP-valienol or other NDP derivatives. In reactions containing 10 mM Mg2+ the enzyme more effectively uses ATP as nucleotidyl donor than GTP or other NTPs
736302
Streptomyces hygroscopicus 5008
?
-
-
-
?
2.7.7.91
UTP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus
diphosphate + UDP-valienol
-
-
-
?
3.1.3.101
trehalose 6-phosphate + H2O
-
736302
Streptomyces hygroscopicus subsp. limoneus
trehalose + phosphate
-
-
-
?
3.1.3.101
validoxylamine A 7'-phosphate + H2O
-
736302
Streptomyces hygroscopicus subsp. limoneus
validoxylamine A + phosphate
validoxylamine A i.e. (1S,2S,3R,6S)-4-(hydroxymethyl)-6-[[(1S,2S,3S,4R,5R)-2,3,4-trihydroxy-5-(hydroxymethyl)cyclohexyl]amino]cyclohex-4-ene-1,2,3-triol
-
-
?
3.1.3.101
validoxylamine A 7'-phosphate + H2O
the enhzyme is involved in the biosynthesis of the antifungal agent validamycin A
736302
Streptomyces hygroscopicus subsp. limoneus
validoxylamine A + phosphate
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.5.1.135
pseudoglycosyltransferase
-
Streptomyces hygroscopicus subsp. limoneus
2.5.1.135
vldE
-
Streptomyces hygroscopicus subsp. limoneus
2.7.7.91
VldB
-
Streptomyces hygroscopicus
3.1.3.101
vldH
-
Streptomyces hygroscopicus subsp. limoneus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5.1.135
7
-
GDP-valienol
at pH 7.5 and 30°C
Streptomyces hygroscopicus subsp. limoneus
2.5.1.135
7
-
validamine 7-phosphate
at pH 7.5 and 30°C
Streptomyces hygroscopicus subsp. limoneus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.135
expressed in Escherichia coli BL21(DE3) cells
Streptomyces hygroscopicus subsp. limoneus
3.1.3.101
expression in Escherichia coli
Streptomyces hygroscopicus subsp. limoneus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.5.1.135
0.0597
-
GDP-valienol
at pH 7.5 and 30°C
Streptomyces hygroscopicus subsp. limoneus
2.5.1.135
0.308
-
validamine 7-phosphate
at pH 7.5 and 30°C
Streptomyces hygroscopicus subsp. limoneus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.91
Mg2+
in reactions containing 1 mM Mg2+, the enzyme effectively catalyzes the conversion of valienol 1-phosphate to GDP-valienol and less effectively to ADP-valienol or other NDP derivatives. In reactions containing 10 mM Mg2+ the enzyme more effectively uses ATP as nucleotidyl donor than GTP or other NTPs
Streptomyces hygroscopicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2.5.1.135
GDP-valienol + validamine 7-phosphate
Streptomyces hygroscopicus subsp. limoneus
-
validoxylamine A 7'-phosphate + GDP
-
-
?
3.1.3.101
validoxylamine A 7'-phosphate + H2O
Streptomyces hygroscopicus subsp. limoneus
the enhzyme is involved in the biosynthesis of the antifungal agent validamycin A
validoxylamine A + phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.5.1.135
Ni-NTA resin column chromatography
Streptomyces hygroscopicus subsp. limoneus
3.1.3.101
-
Streptomyces hygroscopicus subsp. limoneus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.5.1.135
GDP-valienol + validamine 7-phosphate
-
736302
Streptomyces hygroscopicus subsp. limoneus
validoxylamine A 7'-phosphate + GDP
-
-
-
?
2.5.1.135
additional information
incubation of the enzyme with UDP- or GDP-glucose and glucose 6-phosphate does not give any product. No activity with validoxylamine A and UDP-glucose
736302
Streptomyces hygroscopicus subsp. limoneus
?
-
-
-
?
2.7.7.91
ATP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus
diphosphate + ADP-valienol
-
-
-
?
2.7.7.91
ATP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus 5008
diphosphate + ADP-valienol
-
-
-
?
2.7.7.91
CTP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus
diphosphate + CDP-valienol
-
-
-
?
2.7.7.91
CTP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus 5008
diphosphate + CDP-valienol
-
-
-
?
2.7.7.91
dTTP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus
diphosphate + dTDP-valienol
-
-
-
?
2.7.7.91
dTTP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus 5008
diphosphate + dTDP-valienol
-
-
-
?
2.7.7.91
GTP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus
diphosphate + GDP-valienol
-
-
-
?
2.7.7.91
GTP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus 5008
diphosphate + GDP-valienol
-
-
-
?
2.7.7.91
additional information
in reactions containing 1 mM Mg2+, the enzyme effectively catalyzes the conversion of valienol 1-phosphate to GDP-valienol and less effectively to ADP-valienol or other NDP derivatives. In reactions containing 10 mM Mg2+ the enzyme more effectively uses ATP as nucleotidyl donor than GTP or other NTPs
736302
Streptomyces hygroscopicus
?
-
-
-
?
2.7.7.91
additional information
in reactions containing 1 mM Mg2+, the enzyme effectively catalyzes the conversion of valienol 1-phosphate to GDP-valienol and less effectively to ADP-valienol or other NDP derivatives. In reactions containing 10 mM Mg2+ the enzyme more effectively uses ATP as nucleotidyl donor than GTP or other NTPs
736302
Streptomyces hygroscopicus 5008
?
-
-
-
?
2.7.7.91
UTP + valienol 1-phosphate
-
736302
Streptomyces hygroscopicus
diphosphate + UDP-valienol
-
-
-
?
3.1.3.101
trehalose 6-phosphate + H2O
-
736302
Streptomyces hygroscopicus subsp. limoneus
trehalose + phosphate
-
-
-
?
3.1.3.101
validoxylamine A 7'-phosphate + H2O
-
736302
Streptomyces hygroscopicus subsp. limoneus
validoxylamine A + phosphate
validoxylamine A i.e. (1S,2S,3R,6S)-4-(hydroxymethyl)-6-[[(1S,2S,3S,4R,5R)-2,3,4-trihydroxy-5-(hydroxymethyl)cyclohexyl]amino]cyclohex-4-ene-1,2,3-triol
-
-
?
3.1.3.101
validoxylamine A 7'-phosphate + H2O
the enhzyme is involved in the biosynthesis of the antifungal agent validamycin A
736302
Streptomyces hygroscopicus subsp. limoneus
validoxylamine A + phosphate
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.5.1.135
7
-
GDP-valienol
at pH 7.5 and 30°C
Streptomyces hygroscopicus subsp. limoneus
2.5.1.135
7
-
validamine 7-phosphate
at pH 7.5 and 30°C
Streptomyces hygroscopicus subsp. limoneus
General Information
EC Number
General Information
Commentary
Organism
3.1.3.101
metabolism
the enzyme is involved in the biosynthesis of the aminocyclitol antibiotic validamycin A
Streptomyces hygroscopicus subsp. limoneus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.3.101
metabolism
the enzyme is involved in the biosynthesis of the aminocyclitol antibiotic validamycin A
Streptomyces hygroscopicus subsp. limoneus