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Literature summary extracted from

  • Zhao, Z.; Hong, L.; Liu, H.W.
    Characterization of protein encoded by spnR from the spinosyn gene cluster of Saccharopolyspora spinosa: mechanistic implications for forosamine biosynthesis (2005), J. Am. Chem. Soc., 127, 7692-7693.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.110 expressed in Escherichia coli BL21(DE3) cells Saccharopolyspora spinosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.110 dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose + 2-oxoglutarate Saccharopolyspora spinosa
-
 dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.110 Saccharopolyspora spinosa Q9ALN9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.110 Ni-NTA column chromatography and Sephacryl S-200 gel filtration Saccharopolyspora spinosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.110 dTDP-4-amino-2,3,4,6-tetradeoxy-alpha-D-erythro-hexopyranose + 2-oxoglutarate
-
 Saccharopolyspora spinosa dTDP-4-dehydro-2,3,6-trideoxy-alpha-D-hexopyranose + L-glutamate
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.110 homodimer
-
 Saccharopolyspora spinosa

Synonyms

EC Number Synonyms Comment Organism
2.6.1.110 SpnR
-
 Saccharopolyspora spinosa
2.6.1.110 TDP-4-keto-2,3,6-trideoxy-D-glucose 4-aminotransferase
-
 Saccharopolyspora spinosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.110 pyridoxal 5'-phosphate
-
 Saccharopolyspora spinosa