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Literature summary extracted from

  • Ryan, K.L.; Akhmedov, N.G.; Panaccione, D.G.
    Identification and structural elucidation of ergotryptamine, a new ergot alkaloid produced by genetically modified Aspergillus nidulans and natural isolates of Epichloe species (2015), J. Agric. Food Chem., 63, 61-67.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.261 analysis ergot alkaloid pathway reconstruction in Aspergillus nidulans is an approach used to better understand the biosynthesis of these mycotoxins Aspergillus fumigatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.261 recombinant heterologous enzyme expression in Aspergillus nidulans Aspergillus fumigatus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.261 additional information an engineered strain named Aspergillus nidulans WFC, expressing ergot alkaloid synthesis genes dmaW, easF, and easC, produces the established intermediate N-methyl-4-dimethylallyltryptophan, as well as an ergot alkaloid, that is characterized as ergotryptamine, it differs from N-methyl-4-dimethylallyltryptophan by the loss of the carboxyl group, addition of a hydroxyl group, and shift in position of a carbon-carbon double bond, by mass spectrometry, labeling, and NMR. Feeding studies with Aspergillus mutants do not show ergotryptamine turnover, suggesting it is a pathway byproduct as opposed to an authentic intermediate Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.261 S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan Aspergillus fumigatus
-
 S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.261 Aspergillus fumigatus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.261 S-adenosyl-L-methionine + 4-dimethylallyl-L-tryptophan
-
 Aspergillus fumigatus S-adenosyl-L-homocysteine + 4-dimethylallyl-L-abrine
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.261 S-adenosyl-L-methionine
-
 Aspergillus fumigatus