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Literature summary extracted from

  • Guagliardi, A.; Martino, M.; Iaccarino, I.; De Rosa, M.; Rossi, M.; Bartolucci, S.
    Purification and characterization of the alcohol dehydrogenase from a novel strain of Bacillus stearothermophilus growing at 70°C (1996), Int. J. Biochem. Cell Biol., 28, 239-246.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.1 additional information no inhibitory: Triton X-100 at 1%, guanidinium hydrochloride at 0.2 M Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 37000
-
4 * 37000, SDS-PAGE Geobacillus stearothermophilus
1.1.1.1 158000
-
gel filtration Geobacillus stearothermophilus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.1 1-butanol 10%, 29% residual activity Geobacillus stearothermophilus
1.1.1.1 1-propanol 10%, 38% residual activity Geobacillus stearothermophilus
1.1.1.1 Ethanol 10%, 86% residual activity Geobacillus stearothermophilus
1.1.1.1 Methanol 10%, 100% residual activity Geobacillus stearothermophilus
1.1.1.1 urea 0.1 M, 40% residual activity Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Geobacillus stearothermophilus P42328
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 4-methoxybenzaldehyde + NADH + H+
-
Geobacillus stearothermophilus 4-methoxybenzyl alcohol + NAD+ 51% of the activity with butan-2-ol ?
1.1.1.1 acetaldehyde + NADH + H+
-
Geobacillus stearothermophilus ethanol + NAD+ 100% of the activity with butan-2-ol r
1.1.1.1 benzaldehyde + NADH + H+
-
Geobacillus stearothermophilus benzyl alcohol + NAD+ 93% of the activity with butan-2-ol r
1.1.1.1 benzyl alcohol + NAD+
-
Geobacillus stearothermophilus benzaldehyde + NADH + H+ 33% of the activity with butan-2-ol r
1.1.1.1 butan-2-ol + NAD+
-
Geobacillus stearothermophilus butanone + NADH + H+ 83% of the activity with butan-2-ol ?
1.1.1.1 cyclopentanol + NAD+
-
Geobacillus stearothermophilus cyclopentanone + NADH + H+ 92% of the activity with butan-2-ol ?
1.1.1.1 ethanol + NAD+
-
Geobacillus stearothermophilus acetaldehyde + NADH + H+ 83% of the activity with butan-2-ol r
1.1.1.1 pentan-1-ol + NAD+
-
Geobacillus stearothermophilus pentanal + NADH + H+ 76% of the activity with butan-2-ol ?
1.1.1.1 pentan-2-ol + NAD+
-
Geobacillus stearothermophilus pentan-2-one + NADH + H+ 93% of the activity with butan-2-ol ?
1.1.1.1 prop-2-en-1-ol + NAD+
-
Geobacillus stearothermophilus prop-2-enal + NADH + H+ 85% of the activity with butan-2-ol ?
1.1.1.1 propan-1-ol + NAD+
-
Geobacillus stearothermophilus propanal + NADH + H+ 68% of the activity with butan-2-ol r
1.1.1.1 propan-2-ol + NAD+
-
Geobacillus stearothermophilus acetone + NADH + H+ 79% of the activity with butan-2-ol ?
1.1.1.1 propanal + NADH + H+
-
Geobacillus stearothermophilus propan-1-ol + NAD+ 38% of the activity with butan-2-ol r

Subunits

EC Number Subunits Comment Organism
1.1.1.1 tetramer 4 * 37000, SDS-PAGE Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.1 6
-
reduction of acetaldehyde Geobacillus stearothermophilus
1.1.1.1 8
-
oxidation of ethanol Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
Geobacillus stearothermophilus
1.1.1.1 NADH
-
Geobacillus stearothermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.1 Geobacillus stearothermophilus isoelectric focusing
-
4.9