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Literature summary extracted from

  • Kurz, S.; King, J.G.; Dinglasan, R.R.; Paschinger, K.; Wilson, I.B.
    The fucomic potential of mosquitoes: Fucosylated N-glycan epitopes and their cognate fucosyltransferases (2016), Insect Biochem. Mol. Biol., 68, 52-63.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.214 expressed in Pichia pastoris GS115 cells Anopheles gambiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.214 Ca2+ about 45% residual activity at 10 mM; about 75% residual activity at 10 mM Anopheles gambiae
2.4.1.214 Co2+ about 30% residual activity at 10 mM; about 35% residual activity at 10 mM Anopheles gambiae
2.4.1.214 Cu2+ about 28% residual activity at 10 mM; no activity at 10 mM Anopheles gambiae
2.4.1.214 EDTA about 60% residual activity at 10 mM; about 8% residual activity at 10 mM Anopheles gambiae
2.4.1.214 Mg2+ about 45% residual activity at 10 mM; about 70% residual activity at 10 mM Anopheles gambiae
2.4.1.214 Ni2+ about 38% residual activity at 10 mM; about 5% residual activity at 10 mM Anopheles gambiae
2.4.1.214 Zn2+ about 10% residual activity at 10 mM; about 1% residual activity at 10 mM Anopheles gambiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.214 Mn2+ required Anopheles gambiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.214 46000
-
 x * 46000, SDS-PAGE Anopheles gambiae
2.4.1.214 61000
-
 x * 61000, SDS-PAGE Anopheles gambiae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.214 Anopheles gambiae Q7QBF7
-
-
2.4.1.214 Anopheles gambiae Q7QF91
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.214 Ni-NTA column chromatography and Affi-Gel Blue Sepharose column chromatography Anopheles gambiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.214 adult
-
 Anopheles gambiae
-
2.4.1.214 larva
-
 Anopheles gambiae
-

Subunits

EC Number Subunits Comment Organism
2.4.1.214 ? x * 46000, SDS-PAGE Anopheles gambiae
2.4.1.214 ? x * 61000, SDS-PAGE Anopheles gambiae

Synonyms

EC Number Synonyms Comment Organism
2.4.1.214 FucTA
-
 Anopheles gambiae
2.4.1.214 FucTC
-
 Anopheles gambiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.214 22
-
-
 Anopheles gambiae
2.4.1.214 30
-
-
 Anopheles gambiae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.214 10 40 more than 50% activity between 10 and 40°C Anopheles gambiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.214 6 6.5
-
 Anopheles gambiae
2.4.1.214 7 7.5
-
 Anopheles gambiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.214 5 7 more than 50% activity between pH 5.0 and 7.0 Anopheles gambiae
2.4.1.214 6.5 8 more than 50% activity between pH 6.5 and 8.0 Anopheles gambiae