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Literature summary extracted from

  • Zhang, T.; Datta, S.; Eichler, J.; Ivanova, N.; Axen, S.D.; Kerfeld, C.A.; Chen, F.; Kyrpides, N.; Hugenholtz, P.; Cheng,J.-F.; Sale, K.L.; Simmons, B.; Rubin, E.
    Identification of a haloalkaliphilic and thermostable cellulase with improved ionic liquid tolerance (2011), Green Chem., 13, 2083.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.91 extracellular about 39% of the crude enzyme activity Halorhabdus utahensis
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3.2.1.91 soluble about 59% of the crude enzyme activity Halorhabdus utahensis
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Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.91 NaCl enzyme is active in salt concentrations up to 5 M NaCl. The tolerances to heat, alkali and ionic liquids are salt-dependent. At 37°C, the optimal salt concentration is 0.25 M NaCl, at 80°C, the optimal salt concentration is 5 M NaCl Halorhabdus utahensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.91 76000
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 x * 76000, calculated including signal peptide, x * 90000, SDS-PAGE of secreted protein Halorhabdus utahensis
3.2.1.91 90000
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 x * 76000, calculated including signal peptide, x * 90000, SDS-PAGE of secreted protein Halorhabdus utahensis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.91 additional information enzyme is tolerant to high levels (20% w/w) of ionic liquids, including 1-allyl-3-methylimidazolium chloride Halorhabdus utahensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Halorhabdus utahensis C7NVN0
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3.2.1.91 Halorhabdus utahensis DSM 12940 C7NVN0
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Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.91 proteolytic modification the N-terminal region contains the double arginine-based signature of a TAT secretion pathway signal peptide Halorhabdus utahensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O
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 Halorhabdus utahensis 4-nitrophenol + cellobiose
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 ?
3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O
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 Halorhabdus utahensis DSM 12940 4-nitrophenol + cellobiose
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 ?
3.2.1.91 carboxymethyl cellulose + H2O
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 Halorhabdus utahensis cellobiose + ?
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 ?
3.2.1.91 carboxymethyl cellulose + H2O
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 Halorhabdus utahensis DSM 12940 cellobiose + ?
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 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.91 ? x * 76000, calculated including signal peptide, x * 90000, SDS-PAGE of secreted protein Halorhabdus utahensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 Huta_2387 locus name Halorhabdus utahensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.91 additional information
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 the tolerances to heat, alkali and ionic liquids are salt-dependent. At 37°C, the optimal salt concentration is 0.25 M NaCl, at 80°C, the optimal salt concentration is 5 M NaCl Halorhabdus utahensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.91 70
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 presence of 3 M NaCl, 225% of the activity with 2 M NaCl at 37°C Halorhabdus utahensis
3.2.1.91 80
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 presence of 5 M NaCl, 210% of the activity with 2 M NaCl at 37°C Halorhabdus utahensis
3.2.1.91 90
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 presence of 5 M NaCl, about 30% of the activity with 2 M NaCl at 37°C Halorhabdus utahensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.91 9.5
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 presence of 2 M NaCl Halorhabdus utahensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.91 4.5
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 presence of 2 M NaCl, inactive Halorhabdus utahensis
3.2.1.91 7.5 11.5 presence of 2 M NaCl Halorhabdus utahensis

General Information

EC Number General Information Comment Organism
3.2.1.91 physiological function CBH1 is encoded in a gene cluster containing multiple putative cellulolytic enzymes Halorhabdus utahensis