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Literature summary extracted from

  • Uemura, S.; Go, S.; Shishido, F.; Inokuchi, J.
    Expression machinery of GM4: the excess amounts of GM3/GM4S synthase (ST3GAL5) are necessary for GM4 synthesis in mammalian cells (2014), Glycoconj. J., 31, 101-108.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.3.9 expressed in RPMI 1846 or CHO-K1/GalCerS cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.3.9 additional information the cell surface expression of GM4 detected by anti-GM4 antibody is markedly increased in RPMI 1846 cells upon transient transfection of the GM3/GM4S gene, phenotype, overview. Overexpression of the GM3/GM4S gene is required to express GM4 in RPMI 1846 cells Mesocricetus auratus
2.4.3.9 additional information the expression of both the GalCer substrate and endogenous GM3/GM4S is not sufficient to induce GM4 expression in cultured cells. Overexpression of the GM3/GM4S gene is required to express GM4 in CHO-K1/GalCerS cells Cricetulus griseus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.9 endoplasmic reticulum
-
 Mus musculus 5783
-
2.4.3.9 Golgi apparatus
-
 Mus musculus 5794
-
2.4.3.9 Golgi apparatus the enzyme localizes to a compact juxtanuclear reticulum and partially colocalizes with GM130, a cis-Golgi marker protein Homo sapiens 5794
-
2.4.3.9 Golgi apparatus the enzyme localizes to a compact juxtanuclear reticulum and partially colocalizes with GM130, a cis-Golgi marker protein Mus musculus 5794
-
2.4.3.9 Golgi apparatus the enzyme localizes to a compact juxtanuclear reticulum and partially colocalizes with GM130, a cis-Golgi marker protein Mesocricetus auratus 5794
-
2.4.3.9 Golgi apparatus the enzyme localizes to a compact juxtanuclear reticulum and partially colocalizes with GM130, a cis-Golgi marker protein Cricetulus griseus 5794
-
2.4.3.9 membrane
-
 Mus musculus 16020
-
2.4.3.9 additional information immunohistochemic analysis Homo sapiens
-
-
2.4.3.9 additional information immunohistochemic analysis Mus musculus
-
-
2.4.3.9 additional information immunohistochemic analysis Mesocricetus auratus
-
-
2.4.3.9 additional information immunohistochemic analysis Cricetulus griseus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide Homo sapiens
-
 CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide Mus musculus
-
 CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide Mesocricetus auratus
-
 CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide Cricetulus griseus
-
 CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + galactosylceramide Mus musculus
-
 CMP + ganglioside GM3
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + lactosylceramide Mus musculus
-
 CMP + ganglioside GM4
-
 ?
2.4.3.9 CMP-sialic acid + galactosylceramide Homo sapiens
-
 CMP + ganglioside GM4
-
 ?
2.4.3.9 CMP-sialic acid + galactosylceramide Mus musculus
-
 CMP + ganglioside GM4
-
 ?
2.4.3.9 CMP-sialic acid + galactosylceramide Mesocricetus auratus
-
 CMP + ganglioside GM4
-
 ?
2.4.3.9 CMP-sialic acid + galactosylceramide Cricetulus griseus
-
 CMP + ganglioside GM4
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.9 Cricetulus griseus Q80WL0 a mutant lacking galactosylceramide synthase, gene ST3GAL5
-
2.4.3.9 Homo sapiens Q9UNP4 gene ST3GAL5
-
2.4.3.9 Mesocricetus auratus Q9QXF5 gene ST3GAL5
-
2.4.3.9 Mus musculus
-
-
-
2.4.3.9 Mus musculus O88829 gene ST3GAL5
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.9 GM-95 cell
-
 Mus musculus
-
2.4.3.9 HEK-293T cell
-
 Homo sapiens
-
2.4.3.9 kidney
-
 Homo sapiens
-
2.4.3.9 melanoma cell
-
 Mus musculus
-
2.4.3.9 additional information no enzyme activity in CHO-K1 ovary cells lacking or expressing galactosylceramide synthase. The expression of both the GalCer substrate and endogenous GM3/GM4S is not sufficient to induce GM4 expression in cultured cells Cricetulus griseus
-
2.4.3.9 additional information no enzyme activity in RPMI 1846 melanoma cells Mesocricetus auratus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 Homo sapiens CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 Mus musculus CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 Mesocricetus auratus CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 Cricetulus griseus CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + galactosylceramide
-
 Mus musculus CMP + ganglioside GM3
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + lactosylceramide
-
 Mus musculus CMP + ganglioside GM4
-
 ?
2.4.3.9 CMP-sialic acid + galactosylceramide
-
 Homo sapiens CMP + ganglioside GM4
-
 ?
2.4.3.9 CMP-sialic acid + galactosylceramide
-
 Mus musculus CMP + ganglioside GM4
-
 ?
2.4.3.9 CMP-sialic acid + galactosylceramide
-
 Mesocricetus auratus CMP + ganglioside GM4
-
 ?
2.4.3.9 CMP-sialic acid + galactosylceramide
-
 Cricetulus griseus CMP + ganglioside GM4
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.3.9 GM3/GM4S
-
 Mus musculus
2.4.3.9 GM3/GM4S synthase
-
 Mus musculus
2.4.3.9 GM3/GM4S synthase
-
 Homo sapiens
2.4.3.9 GM3/GM4S synthase
-
 Mesocricetus auratus
2.4.3.9 GM3/GM4S synthase
-
 Cricetulus griseus
2.4.3.9 ST3Gal5
-
 Mus musculus
2.4.3.9 ST3Gal5
-
 Homo sapiens
2.4.3.9 ST3Gal5
-
 Mesocricetus auratus
2.4.3.9 ST3Gal5
-
 Cricetulus griseus