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Literature summary extracted from

  • Wu, Z.; Zhao, G.; Li, T.; Qu, J.; Guan, W.; Wang, J.; Ma, C.; Li, X.; Zhao, W.; Wang, P.G.; Li, L.
    Biochemical characterization of an alpha1,2-colitosyltransferase from Escherichia coli O55:H7 (2015), Glycobiology, 26, 493-500.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.341 the gene cloned into pQE80 L for expression Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.341 0.76
-
 GDP-beta-L-colitose pH 8.0, 37°C Escherichia coli
2.4.1.341 1.3
-
 GDP-L-fucose pH 8.0, 37°C Escherichia coli
2.4.1.341 1.4
-
 beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine pH 8.0, 37°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.341 membrane
-
 Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.341 additional information the enzyme does not require a metal ion Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.341 30000
-
 x * 30000, N-terminal Histagged, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.341 GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine Escherichia coli beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme participates in the biosynthesis of an O-antigen GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
-
 ?
2.4.1.341 GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine Escherichia coli O55:H5 beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme participates in the biosynthesis of an O-antigen GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.341 Escherichia coli
-
-
-
2.4.1.341 Escherichia coli O55:H5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.341
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.341 GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme participates in the biosynthesis of an O-antigen Escherichia coli GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
-
 ?
2.4.1.341 GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme is highly specific towards the acceptor, exclusively recognizing lacto-N-biose, but can accept GDP-L-fucose as the donor with almost the same activity as with GDP-beta-L-colitose Escherichia coli GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
-
 ?
2.4.1.341 GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme participates in the biosynthesis of an O-antigen Escherichia coli O55:H5 GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
-
 ?
2.4.1.341 GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme is highly specific towards the acceptor, exclusively recognizing lacto-N-biose, but can accept GDP-L-fucose as the donor with almost the same activity as with GDP-beta-L-colitose Escherichia coli O55:H5 GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
-
 ?
2.4.1.341 GDP-L-fucose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme is highly specific towards the acceptor, exclusively recognizing lacto-N-biose, but can accept GDP-L-fucose as the donor with almost the same activity as with GDP-beta-L-colitose Escherichia coli GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
-
 ?
2.4.1.341 GDP-L-fucose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme is highly specific towards the acceptor, exclusively recognizing lacto-N-biose, but can accept GDP-L-fucose as the donor with almost the same activity as with GDP-beta-L-colitose Escherichia coli O55:H5 GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.341 ? x * 30000, N-terminal Histagged, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.4.1.341 alpha1,2-ColT
-
 Escherichia coli
2.4.1.341 wbgN
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.341 0.087
-
 beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine pH 8.0, 37°C Escherichia coli
2.4.1.341 0.147
-
 GDP-beta-L-colitose pH 8.0, 37°C Escherichia coli
2.4.1.341 0.2
-
 GDP-L-fucose pH 8.0, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.341 7.5 9
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.341 10.2
-
 about 60% of maximal activity Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.341 0.062
-
 beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine pH 8.0, 37°C Escherichia coli
2.4.1.341 0.15
-
 GDP-L-fucose pH 8.0, 37°C Escherichia coli
2.4.1.341 0.193
-
 GDP-beta-L-colitose pH 8.0, 37°C Escherichia coli