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Literature summary extracted from

  • Ara, K.Z.; Lundemo, P.; Fridjonsson, O.H.; Hreggvidsson, G.O.; Adlercreutz, P.; Karlsson, E.N.
    A CGTase with high coupling activity using gamma-cyclodextrin isolated from a novel strain clustering under the genus Carboxydocella (2015), Glycobiology, 25, 514-523.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 expressed in Escherichia coli BL21(DE3) cells uncultured Carboxydocella sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.19 60000
-
 x * 60000, SDS-PAGE uncultured Carboxydocella sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.19 potato starch + glycosyl acceptor uncultured Carboxydocella sp. worst substrate alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 potato starch + glycosyl acceptor uncultured Carboxydocella sp. MAT3016 worst substrate alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor uncultured Carboxydocella sp.
-
 alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor uncultured Carboxydocella sp. MAT3016
-
 alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 wheat starch + glycosyl acceptor uncultured Carboxydocella sp. best substrate, the enzyme is preferentially forming alpha- (50%) and beta-cyclodextrin (40%) in the cyclization reaction using wheat starch as substrate alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 wheat starch + glycosyl acceptor uncultured Carboxydocella sp. MAT3016 best substrate, the enzyme is preferentially forming alpha- (50%) and beta-cyclodextrin (40%) in the cyclization reaction using wheat starch as substrate alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 uncultured Carboxydocella sp.
-
-
-
2.4.1.19 uncultured Carboxydocella sp. MAT3016
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 HisTrap column chromatography, and gel filtration uncultured Carboxydocella sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 potato starch + glycosyl acceptor worst substrate uncultured Carboxydocella sp. alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 potato starch + glycosyl acceptor worst substrate uncultured Carboxydocella sp. MAT3016 alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 uncultured Carboxydocella sp. alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 uncultured Carboxydocella sp. MAT3016 alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 wheat starch + glycosyl acceptor best substrate, the enzyme is preferentially forming alpha- (50%) and beta-cyclodextrin (40%) in the cyclization reaction using wheat starch as substrate uncultured Carboxydocella sp. alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?
2.4.1.19 wheat starch + glycosyl acceptor best substrate, the enzyme is preferentially forming alpha- (50%) and beta-cyclodextrin (40%) in the cyclization reaction using wheat starch as substrate uncultured Carboxydocella sp. MAT3016 alpha-cyclodextrin + beta-cyclodextrin + gamma-cyclodextrin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.19 ? x * 60000, SDS-PAGE uncultured Carboxydocella sp.

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 CGT13
-
 uncultured Carboxydocella sp.
2.4.1.19 CGTase
-
 uncultured Carboxydocella sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 65
-
-
 uncultured Carboxydocella sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 6
-
-
 uncultured Carboxydocella sp.