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Literature summary extracted from

  • Alam, S.; Fedier, A.; Kohler, R.S.; Jacob, F.
    Glucosylceramide synthase inhibitors differentially affect expression of glycosphingolipids (2015), Glycobiology, 25, 351-356.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.80 DL-erythro-1-phenyl-2-decanoylamino-3-morpholino-1-propanol the enzyme GCS inhibitor causes cytotoxic, antiproliferative, antiangiogenic and tumor-volume-reducing effects, modulating drug responses in cell lines both cytodestructive (as a drug sensitizer) and cytoprotective, effect on cell survival, overview. The inhibitor is not suitable to deplete cells of the neolacto glycosphingolipid series Homo sapiens
2.4.1.80 DL-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol the enzyme GCS inhibitor causes cytotoxic, antiproliferative, antiangiogenic and tumor-volume-reducing effects, modulating drug responses in cell lines both cytodestructive (as a drug sensitizer) and cytoprotective, effect on cell survival, overview. The inhibitor is not suitable to deplete cells of the neolacto glycosphingolipid series Homo sapiens
2.4.1.80 additional information GCS enzyme inhibitors affect the expression of lactosylceramide, neolacto, ganglio, and globo series glycosphingolipids in a panel of human breast cancer cell lines using flow cytometry, a commonly applied method investigating cell-surface glycosphingolipids after enzyme inhibition, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.80 UDP-glucose + an N-acylsphingosine Homo sapiens
-
 UDP + a D-glucosyl-N-acylsphingosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.80 Homo sapiens Q16739
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.80 breast cancer cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.80 UDP-glucose + an N-acylsphingosine
-
 Homo sapiens UDP + a D-glucosyl-N-acylsphingosine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.80 GCS
-
 Homo sapiens
2.4.1.80 glucosylceramide synthase
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.1.80 malfunction enzyme inhibitors affect the expression of lactosylceramide, neolacto, ganglio, and globo series glycosphingolipids in a panel of human cancer cell lines. Their cell-surface glycosphingolipid expression considerably varies among cell lines and sublethal concentrations (IC10) of both inhibitors preferentially and significantly reduce the expression of globo Gb3 glycosphingolipids in the cancer cell lines IGROV1, BG1, HT29 and T47D, whereas globo SSEA3 glycosphingolipid expression is only reduced in BG1 cells. The neolacto and ganglio glycosphingolipid series is not affected. Lactosylceramider, the precursor of all GlcCer-related glycosphingolipids, is significantly reduced only in BG1 cells treated with DL-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol, overview Homo sapiens
2.4.1.80 metabolism glucosylceramide synthase catalyzes the first committed step in the biosynthesis of glucosylceramide-related glycosphingolipids, pathway overview Homo sapiens