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Literature summary extracted from

  • Abdel Rahman, A.; Ryczko, M.; Nakano, M.; Pawling, J.; Rodrigues, T.; Johswich, A.; Taniguchi, N.; Dennis, J.
    Golgi N-glycan branching N-acetylglucosaminyltransferases I, V and VI promote nutrient uptake and metabolism (2015), Glycobiology, 25, 225-240.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.101 recombinant tet-inducible expression of Flag-tagged GlcNAcT-I in HeLa and HEK293 cells Homo sapiens
2.4.1.155 gene MGAT5, recombinant tet-inducible expression of Flag-tagged GlcNAcT-V in HeLa and HEK293 cells. Tet-induced Mgat5 HEK293 cells display a 2fold increase in tri-antennary and a 2fold increase in tetraantennary N-glycans Homo sapiens
2.4.1.201 gene MGAT6, recombinant tet-inducible expression of Flag-tagged GlcNAcT-V in HeLa and HEK293 cells. Tet-induced Mgat6 HEK293 cells produce increased oligosaccharide levels also observed in HeLa cells, predicted to be the 2,2,4,6 tetra-, 4,2,2,4 tetra- and 4,2,2,4,6 penta-N-glycans, while native 4,2,2,6 tetra are reduced, N-glycans profiles of transgenic HeLa and HEK-293 cells, overview Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.101 additional information GlcNAc and tet-induced Mgat1 increases the levels of many metabolites in HeLa cells, and HEK293 cells, tet-induced Mgat1 changes in the N-glycan distributions, , N-glycans profiles of transgenic HeLa and HEK-293 cells, overview Homo sapiens
2.4.1.155 additional information GlcNAc and tet-induced Mgat5 increases the levels of many metabolites in HeLa cells, and HEK293 cells, and tet-induced Mgat5 changes in the N-glycan distributions, N-glycans profiles of transgenic HeLa and HEK-293 cells, overview. Tet-inducible Mgat5 enhances amino acid uptake and growth in nutrient-poor conditions and complex-type N-glycan branching in HEK293 Mgat5 clones. Up-regulation of Mgat5 in HEK293 cells stimulates amino acid uptake and increases metabolite levels under low Gln/Glc culture conditions Homo sapiens
2.4.1.201 additional information GlcNAc and tet-induced Mgat6 increases the levels of many metabolites in HeLa cells, and HEK293 cells, and tet-induced Mgat5 changes in the N-glycan distributions, N-glycans profiles of transgenic HeLa cells, overview. Tet-inducible Mgat5 enhances amino acid uptake and growth in nutrient-poor conditions. The Mgat5, EC 2.4.1.155, product is the preferred acceptor for Mgat6, which adds a GlcNAcbeta1,4 branch that is not present in mammals, Mgat6 enhances functionality of N-glycan branching in mammalian cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.101 Golgi apparatus
-
 Homo sapiens 5794
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.101 53000
-
 x * 53000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.101 UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R Homo sapiens
-
 UDP + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?
2.4.1.155 UDP-N-acetyl-D-glucosamine + 6-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R Homo sapiens
-
 UDP + 6-(2,6-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?
2.4.1.201 UDP-N-acetyl-D-glucosamine + 2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R Homo sapiens
-
 UDP + 2,4,6-tris(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.101 Homo sapiens P26572
-
-
2.4.1.155 Homo sapiens Q09328 gene MGAT5
-
2.4.1.201 Homo sapiens
-
 gene MGAT6
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.101 UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R
-
 Homo sapiens UDP + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?
2.4.1.155 UDP-N-acetyl-D-glucosamine + 6-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 Homo sapiens UDP + 6-(2,6-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?
2.4.1.201 UDP-N-acetyl-D-glucosamine + 2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R
-
 Homo sapiens UDP + 2,4,6-tris(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl-R
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.101 ? x * 53000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.101 GlcNAcT-I
-
 Homo sapiens
2.4.1.101 Golgi N-glycan branching N-acetylglucosaminyltransferase I
-
 Homo sapiens
2.4.1.101 MGAT1
-
 Homo sapiens
2.4.1.155 GnT-V
-
 Homo sapiens
2.4.1.155 Mgat5
-
 Homo sapiens
2.4.1.155 N-acetylglucosaminyltransferase V
-
 Homo sapiens
2.4.1.201 GCNT6
-
 Homo sapiens
2.4.1.201 GnT-VI
-
 Homo sapiens
2.4.1.201 Mgat6
-
 Homo sapiens
2.4.1.201 N-acetylglucosaminyltransferase VI
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.101 37
-
 assay at Homo sapiens
2.4.1.155 37
-
 assay at Homo sapiens
2.4.1.201 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.101 6.5
-
 assay at Homo sapiens
2.4.1.155 6.5
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
2.4.1.101 metabolism the enzyme is involved in the N-glycan-branching pathway, overview. Regulation of cellular metabolite levels by Mgat1, overview Homo sapiens
2.4.1.155 metabolism the enzyme is involved in the N-glycan-branching pathway, regulation of cellular metabolite levels by Mgat5, overview Homo sapiens
2.4.1.201 metabolism the enzyme is involved in the N-glycan-branching pathway, regulation of cellular metabolite levels by Mgat6, overview Homo sapiens