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Literature summary extracted from

  • Engels, L.; Elling, L.
    WbgL: A novel bacterial alpha1,2-fucosyltransferase for the synthesis of 2-fucosyllactose (2014), Glycobiology, 24, 170-178.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.69 expressed in Escherichia coli BL21(DE3) and JM109 (DE3) cells Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.69 Co2+ less than 20% residual activity at 2 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.69 0.27
-
GDP-beta-L-fucose at pH 7.6 and 30°C Escherichia coli
2.4.1.69 5.3
-
D-lactose at pH 7.6 and 30°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.69 additional information the enzyme shows no strict requirement for divalent metal ions Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.69 59000
-
x * 59000, calculated from amino acid sequence Escherichia coli
2.4.1.69 70000
-
x * 70000, recombinant, His- and pro-protein-tagged enzyme, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.69 GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide Escherichia coli
-
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
?
2.4.1.69 GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide Escherichia coli O126
-
GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.69 Escherichia coli A6M9C2
-
-
2.4.1.69 Escherichia coli O126 A6M9C2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.69 Ni2+-NTA column chromatography Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.4.1.69 -20°C, 50 mM Tris-HCl buffer pH 7.6, 2 months, 30% loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.69 GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
Escherichia coli GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
?
2.4.1.69 GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
Escherichia coli O126 GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
?
2.4.1.69 GDP-beta-L-fucose + D-galactose 70% activity compared to D-lactose Escherichia coli ?
-
?
2.4.1.69 GDP-beta-L-fucose + D-galactose 70% activity compared to D-lactose Escherichia coli O126 ?
-
?
2.4.1.69 GDP-beta-L-fucose + D-lactose 100% activity Escherichia coli GDP + 2'-fucosyl-D-lactose
-
?
2.4.1.69 GDP-beta-L-fucose + D-lactose 100% activity Escherichia coli O126 GDP + 2'-fucosyl-D-lactose
-
?
2.4.1.69 GDP-beta-L-fucose + D-lactulose 125% activity compared to D-lactose Escherichia coli GDP + 2'-fucosyl-D-lactulose
-
?
2.4.1.69 GDP-beta-L-fucose + D-lactulose 125% activity compared to D-lactose Escherichia coli O126 GDP + 2'-fucosyl-D-lactulose
-
?
2.4.1.69 GDP-beta-L-fucose + Gal(beta1-3)GlcNAc 28% activity compared to D-lactose Escherichia coli ?
-
?
2.4.1.69 GDP-beta-L-fucose + Gal(beta1-4)GlcNAc 85% activity compared to D-lactose Escherichia coli ?
-
?
2.4.1.69 GDP-beta-L-fucose + Galbeta-4-nitrophenyl 28% activity compared to D-lactose Escherichia coli ?
-
?
2.4.1.69 additional information no activity with D-galactosamine, N-acetyl-D-galactosamine, Gal(beta1-4)Glcbeta1-Bn, Gal(beta1-4)Glcbeta1-octyl, Gal(beta1-4)[Fuc(alpha1-3)]Glc, Gal(beta1-4)GlcNAc(beta1-3)Gal(beta1-4)GlcNAcbeta-linker-tBoc, and Gal(alpha1-6)Glc(alpha1-2)Fru Escherichia coli ?
-
?
2.4.1.69 additional information no activity with D-galactosamine, N-acetyl-D-galactosamine, Gal(beta1-4)Glcbeta1-Bn, Gal(beta1-4)Glcbeta1-octyl, Gal(beta1-4)[Fuc(alpha1-3)]Glc, Gal(beta1-4)GlcNAc(beta1-3)Gal(beta1-4)GlcNAcbeta-linker-tBoc, and Gal(alpha1-6)Glc(alpha1-2)Fru Escherichia coli O126 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.69 ? x * 59000, calculated from amino acid sequence Escherichia coli
2.4.1.69 ? x * 70000, recombinant, His- and pro-protein-tagged enzyme, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.4.1.69 alpha1,2-fucosyltransferase
-
Escherichia coli
2.4.1.69 alpha1,2-FucT
-
Escherichia coli
2.4.1.69 WbgL
-
Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.69 27
-
at 27°C in 50 mM Tris-HCl buffer pH 7.6, the enzyme shows a half -life of 57 h Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.69 0.066
-
GDP-beta-L-fucose at pH 7.6 and 30°C Escherichia coli
2.4.1.69 0.067
-
D-lactose at pH 7.6 and 30°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.69 7.6
-
-
Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.69 7 8
-
Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.69 Escherichia coli calculated from amino acid sequence
-
6.2

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.69 0.013
-
D-lactose at pH 7.6 and 30°C Escherichia coli
2.4.1.69 0.245
-
GDP-beta-L-fucose at pH 7.6 and 30°C Escherichia coli