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Literature summary extracted from

  • Van der Sluis, R.; Badenhorst, C.; Van der Westhuizen, F.; Van Dijk, A.
    Characterisation of the influence of genetic variations on the enzyme activity of a recombinant human glycine N-acyltransferase (2013), Gene, 515, 447-453.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.13 expressed in Escherichia coli Origami cells Homo sapiens
2.3.1.71 expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.71 molecular model containing coenzyme A. The inactivity of the R199C variant could be due to the substitution of the highly conserved Arg199 and destabilisation of an alpha-loop-alpha motif which is important for substrate binding Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.13 E227Q inactive Homo sapiens
2.3.1.13 F168L the mutant shows decreased activity compared to the wild type enzyme Homo sapiens
2.3.1.13 K16N the mutant shows wild type activity Homo sapiens
2.3.1.13 N156S the mutant shows increased activity compared to the wild type enzyme Homo sapiens
2.3.1.13 R131H the mutant shows wild type activity Homo sapiens
2.3.1.13 R199C inactive Homo sapiens
2.3.1.13 S17T the mutant shows wild type activity Homo sapiens
2.3.1.71 E229Q single nucleotide polymorphism, inactive Homo sapiens
2.3.1.71 F168L single nucleotide polymorphism, decreased activity compared to wild-type Homo sapiens
2.3.1.71 K16N single nucleotide polymorphism, enzymic activities similar to wild-type Homo sapiens
2.3.1.71 N156S single nucleotide polymorphism, more active than wild-type Homo sapiens
2.3.1.71 R131H single nucleotide polymorphism, enzymic activities similar to wild-type Homo sapiens
2.3.1.71 R199C single nucleotide polymorphism, inactive Homo sapiens
2.3.1.71 S17T single nucleotide polymorphism, enzymic activities similar to wild-type Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.13 0.021
-
 benzoyl-CoA mutant enzyme K16N, apparent value, at pH 8.0 and 37°C Homo sapiens
2.3.1.13 0.024
-
 benzoyl-CoA wild type enzyme, apparent value, at pH 8.0 and 37°C Homo sapiens
2.3.1.13 0.028
-
 benzoyl-CoA mutant enzyme S17T, apparent value, at pH 8.0 and 37°C Homo sapiens
2.3.1.13 0.038
-
 benzoyl-CoA mutant enzyme N156S, apparent value, at pH 8.0 and 37°C Homo sapiens
2.3.1.13 0.053
-
 benzoyl-CoA mutant enzyme F168L, apparent value, at pH 8.0 and 37°C Homo sapiens
2.3.1.13 0.071
-
 benzoyl-CoA mutant enzyme R131H, apparent value, at pH 8.0 and 37°C Homo sapiens
2.3.1.71 0.021
-
 benzoyl-CoA mutant K16N, pH 8.0, 37°C Homo sapiens
2.3.1.71 0.024
-
 benzoyl-CoA wild-type, pH 8.0, 37°C Homo sapiens
2.3.1.71 0.028
-
 benzoyl-CoA mutant S17T, pH 8.0, 37°C Homo sapiens
2.3.1.71 0.038
-
 benzoyl-CoA mutant N156S, pH 8.0, 37°C Homo sapiens
2.3.1.71 0.053
-
 benzoyl-CoA mutant F168L, pH 8.0, 37°C Homo sapiens
2.3.1.71 0.071
-
 benzoyl-CoA mutant R131H, pH 8.0, 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.13 56000
-
 x * 56000, thioredoxin-His-tagged fusion protein, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.13 Homo sapiens Q6IB77
-
-
2.3.1.71 Homo sapiens Q6IB77
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.13 Protino Ni-TED 2000 column chromatography and Sephadex G25 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.13 liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.13 benzoyl-CoA + glycine
-
 Homo sapiens CoA + N-benzoylglycine
-
 ?
2.3.1.71 benzoyl-CoA + glycine
-
 Homo sapiens CoA + N-benzoylglycine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.13 ? x * 56000, thioredoxin-His-tagged fusion protein, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.13 GlyAT
-
 Homo sapiens