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Literature summary extracted from

  • Duffner, F.M.; Kirchner, U.; Bauer, M.P.; Mueller, R.
    Phenol/cresol degradation by the thermophilic Bacillus thermoglucosidasius A7: cloning and sequence analysis of five genes involved in the pathway (2000), Gene, 256, 215-221.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.20 gene pheA1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli, coexpression with pheA2 is required for catalytic activity Parageobacillus thermoglucosidasius
1.14.14.20 gene pheA2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli, coexpression with pheA1 is required for catalytic activity Parageobacillus thermoglucosidasius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.20 intracellular
-
 Parageobacillus thermoglucosidasius 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.20 18000
-
 x * 18000, about, sequence calculation Parageobacillus thermoglucosidasius
1.14.14.20 57000
-
 x * 57000, about, sequence calculation Parageobacillus thermoglucosidasius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.20 phenol + FADH2 + O2 Parageobacillus thermoglucosidasius
-
 catechol + FAD + H2O
-
 ?
1.14.14.20 phenol + FADH2 + O2 Parageobacillus thermoglucosidasius A7
-
 catechol + FAD + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.20 Parageobacillus thermoglucosidasius Q9LAG2 PheA2; or Bacillus thermoglucosidasius, gene pheA2
-
1.14.14.20 Parageobacillus thermoglucosidasius Q9LAG3 PheA1; or Bacillus thermoglucosidasius, gene pheA1
-
1.14.14.20 Parageobacillus thermoglucosidasius A7 Q9LAG2 PheA2; or Bacillus thermoglucosidasius, gene pheA2
-
1.14.14.20 Parageobacillus thermoglucosidasius A7 Q9LAG3 PheA1; or Bacillus thermoglucosidasius, gene pheA1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.20 phenol + FADH2 + O2
-
 Parageobacillus thermoglucosidasius catechol + FAD + H2O
-
 ?
1.14.14.20 phenol + FADH2 + O2
-
 Parageobacillus thermoglucosidasius A7 catechol + FAD + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.20 ? x * 18000, about, sequence calculation Parageobacillus thermoglucosidasius
1.14.14.20 ? x * 57000, about, sequence calculation Parageobacillus thermoglucosidasius

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.20 FADH2
-
 Parageobacillus thermoglucosidasius

General Information

EC Number General Information Comment Organism
1.14.14.20 physiological function Geobacillus thermoglucosidasius strain A7 degrades phenol at 65°C via the meta cleavage pathway Parageobacillus thermoglucosidasius