BRENDA - Enzyme Database

Differential regulation of two types of monogalactosyldiacylglycerol synthase in membrane lipid remodeling under phosphate-limited conditions in sesame plants

Shimojima, M.; Watanabe, T.; Madoka, Y.; Koizumi, R.; Yamamoto, M.P.; Masuda, K.; Yamada, K.; Masuda, S.; Ohta, H.; Front. Plant Sci. 4, 469 (2013)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.46
phosphatidic acid
;
Sesamum indicum
Cloned(Commentary)
EC Number
Commentary
Organism
2.4.1.46
expressed in Escherichia coli BL21(DE3) cells; expressed in Escherichia coli BL21(DE3) cells
Sesamum indicum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.46
chloroplast
outer envelope membrane of chloroplasts; outer envelope membrane of chloroplasts
Sesamum indicum
9507
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.46
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol
Sesamum indicum
-
UDP + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
-
?
2.4.1.46
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol
Sesamum indicum 4294
-
UDP + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.46
Sesamum indicum
S6B542
-
-
2.4.1.46
Sesamum indicum
S6BK49
-
-
2.4.1.46
Sesamum indicum 4294
S6B542
-
-
2.4.1.46
Sesamum indicum 4294
S6BK49
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
2.4.1.46
both isoform MGD1 and MGD2 are inactivated by oxidization
Sesamum indicum
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.46
Ni-NTA agarose column chromatography, and gel filtration; Ni-NTA agarose column chromatography, and gel filtration
Sesamum indicum
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.46
shoot
;
Sesamum indicum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.46
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol
-
736153
Sesamum indicum
UDP + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
-
-
?
2.4.1.46
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol
-
736153
Sesamum indicum 4294
UDP + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.46
phosphatidic acid
-
Sesamum indicum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.46
expressed in Escherichia coli BL21(DE3) cells
Sesamum indicum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.46
chloroplast
outer envelope membrane of chloroplasts
Sesamum indicum
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.46
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol
Sesamum indicum
-
UDP + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
-
?
2.4.1.46
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol
Sesamum indicum 4294
-
UDP + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
-
?
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
2.4.1.46
both isoform MGD1 and MGD2 are inactivated by oxidization
Sesamum indicum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.46
Ni-NTA agarose column chromatography, and gel filtration
Sesamum indicum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.46
shoot
-
Sesamum indicum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.46
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol
-
736153
Sesamum indicum
UDP + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
-
-
?
2.4.1.46
UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol
-
736153
Sesamum indicum 4294
UDP + 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol
-
-
-
?