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Literature summary extracted from

  • Leis, B.; Heinze, S.; Angelov, A.; Pham, V.T.; Thuermer, A.; Jebbar, M.; Golyshin, P.N.; Streit, W.R.; Daniel, R.; Liebl, W.
    Functional screening of hydrolytic activities reveals an extremely thermostable cellulase from a deep-sea archaeon (2015), Front. Bioeng. Biotechnol., 3, 95.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 dithiothreitol improves enzymatic activity by 65.9-146.2% when added at final concentrations of 10 and 1.0 mM, respectively uncultured bacterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Escherichia coli uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 carboxymethyl cellulose Km-value is 2.35 mg/ml, pH 5.5, 92°C uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 CoCl2 0.5 mM, 210% of initial activity uncultured bacterium
3.2.1.4 KCl 500 mM, 75% increase in activity uncultured bacterium
3.2.1.4 MnCl2 1-2 mM, 188% of initial activity uncultured bacterium
3.2.1.4 NaCl 500 mM, 75% increase in activity uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 60000
-
 x * 62300, calculated, x * 60000, SDS-PAGE uncultured bacterium
3.2.1.4 62300
-
 x * 62300, calculated, x * 60000, SDS-PAGE uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 uncultured bacterium A0A0F7YYA5 isolates of hyperthermophilic archaea from deep sea vents
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 34
-
 substrate phosphoric acid-swollen cellulose, pH 5.5, 92°C uncultured bacterium
3.2.1.4 108
-
 substrate hydroxyethyl cellulose, pH 5.5, 92°C uncultured bacterium
3.2.1.4 272
-
 substrate lichenan, pH 5.5, 92°C uncultured bacterium
3.2.1.4 317
-
 substrate barley beta-glucan, pH 5.5, 92°C uncultured bacterium
3.2.1.4 692
-
 substrate carboxymethyl cellulose, pH 5.5, 92°C uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 barley beta-glucan + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.4 hydroxyethyl cellulose + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.4 lichenan + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.4 additional information enzyme displays highest specific activity against beta-1,4-glycosidic bonds of various linear glucan polysaccharides. Poor or no substrates: avicel, pachyman, laminarin, starch uncultured bacterium ?
-
 ?
3.2.1.4 phosphoric acid-swollen cellulose + H2O
-
 uncultured bacterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 62300, calculated, x * 60000, SDS-PAGE uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Cel12A
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 90 95
-
 uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5.5
-
-
 uncultured bacterium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.4 uncultured bacterium calculated
-
 4.3