BRENDA - Enzyme Database

Gene redundancy of two-component (chloro)phenol hydroxylases in Rhodococcus opacus 1CP

Groening, J.; Eulberg, D.; Tischler, D.; Kaschabek, S.; Schloemann, M.; FEMS Microbiol. Lett. 361, 68-75 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.14.20
genes pheA1(1-3), phylogenetic analysis, typical for genes of the peripheral degradation of aromatic compounds, pheA1(1–3) and pheA2(1-3) are not located within gene clusters for central ortho- and meta-cleavage pathway. All three gene sets are nearby to genes with function in (chloro)aromatic degradation
Rhodococcus opacus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.20
290000
310000
isozymes, gel filtration
Rhodococcus opacus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.20
2,4-dichlorophenol + FADH2 + O2
Rhodococcus opacus
-
2,4-dichlorocatechol + FAD + H2O
-
-
?
1.14.14.20
2,4-dichlorophenol + FADH2 + O2
Rhodococcus opacus 1CP
-
2,4-dichlorocatechol + FAD + H2O
-
-
?
1.14.14.20
2-chlorophenol + FADH2 + O2
Rhodococcus opacus
poor substrate for isozyme PheA1(1)
2-chlorocatechol + FAD + H2O
-
-
?
1.14.14.20
2-chlorophenol + FADH2 + O2
Rhodococcus opacus 1CP
poor substrate for isozyme PheA1(1)
2-chlorocatechol + FAD + H2O
-
-
?
1.14.14.20
4-chlorophenol + FADH2 + O2
Rhodococcus opacus
-
4-chlorocatechol + FAD + H2O
-
-
?
1.14.14.20
4-chlorophenol + FADH2 + O2
Rhodococcus opacus 1CP
-
4-chlorocatechol + FAD + H2O
-
-
?
1.14.14.20
4-methylphenol + FADH2 + O2
Rhodococcus opacus
-
4-methylcatechol + FAD + H2O
-
-
?
1.14.14.20
additional information
Rhodococcus opacus
substrate speccificities of the three isozymes, overview
?
-
-
-
1.14.14.20
additional information
Rhodococcus opacus 1CP
substrate speccificities of the three isozymes, overview
?
-
-
-
1.14.14.20
phenol + FADH2 + O2
Rhodococcus opacus
-
catechol + FAD + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.20
Rhodococcus opacus
A0A069AW73
gene pheA1(1); gene pheA1(1)
-
1.14.14.20
Rhodococcus opacus 1CP
A0A069AW73
gene pheA1(1); gene pheA1(1)
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.20
2,4-dichlorophenol + FADH2 + O2
-
736121
Rhodococcus opacus
2,4-dichlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
2,4-dichlorophenol + FADH2 + O2
-
736121
Rhodococcus opacus 1CP
2,4-dichlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
2-chlorophenol + FADH2 + O2
-
736121
Rhodococcus opacus
2-chlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
2-chlorophenol + FADH2 + O2
poor substrate for isozyme PheA1(1)
736121
Rhodococcus opacus
2-chlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
2-chlorophenol + FADH2 + O2
-
736121
Rhodococcus opacus 1CP
2-chlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
2-chlorophenol + FADH2 + O2
poor substrate for isozyme PheA1(1)
736121
Rhodococcus opacus 1CP
2-chlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
4-chlorophenol + FADH2 + O2
-
736121
Rhodococcus opacus
4-chlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
4-chlorophenol + FADH2 + O2
-
736121
Rhodococcus opacus 1CP
4-chlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
4-methylphenol + FADH2 + O2
-
736121
Rhodococcus opacus
4-methylcatechol + FAD + H2O
-
-
-
?
1.14.14.20
additional information
substrate speccificities of the three isozymes, overview
736121
Rhodococcus opacus
?
-
-
-
-
1.14.14.20
additional information
substrate speccificities of the three isozymes, overview
736121
Rhodococcus opacus 1CP
?
-
-
-
-
1.14.14.20
phenol + FADH2 + O2
-
736121
Rhodococcus opacus
catechol + FAD + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.14.20
oligomer
homotetrameric or homohexameric structure, all phenol hydroxylase isoenzymes, x * 59000-63000, SDS-PAGE
Rhodococcus opacus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.14.20
25
-
assay at
Rhodococcus opacus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.20
7.2
-
assay at
Rhodococcus opacus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.20
FADH2
FAD is again reduced at the expense of NADH and NADPH
Rhodococcus opacus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.20
genes pheA1(1-3), phylogenetic analysis, typical for genes of the peripheral degradation of aromatic compounds, pheA1(1–3) and pheA2(1-3) are not located within gene clusters for central ortho- and meta-cleavage pathway. All three gene sets are nearby to genes with function in (chloro)aromatic degradation
Rhodococcus opacus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.20
FADH2
FAD is again reduced at the expense of NADH and NADPH
Rhodococcus opacus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.14.20
290000
310000
isozymes, gel filtration
Rhodococcus opacus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.20
2,4-dichlorophenol + FADH2 + O2
Rhodococcus opacus
-
2,4-dichlorocatechol + FAD + H2O
-
-
?
1.14.14.20
2,4-dichlorophenol + FADH2 + O2
Rhodococcus opacus 1CP
-
2,4-dichlorocatechol + FAD + H2O
-
-
?
1.14.14.20
2-chlorophenol + FADH2 + O2
Rhodococcus opacus
poor substrate for isozyme PheA1(1)
2-chlorocatechol + FAD + H2O
-
-
?
1.14.14.20
2-chlorophenol + FADH2 + O2
Rhodococcus opacus 1CP
poor substrate for isozyme PheA1(1)
2-chlorocatechol + FAD + H2O
-
-
?
1.14.14.20
4-chlorophenol + FADH2 + O2
Rhodococcus opacus
-
4-chlorocatechol + FAD + H2O
-
-
?
1.14.14.20
4-chlorophenol + FADH2 + O2
Rhodococcus opacus 1CP
-
4-chlorocatechol + FAD + H2O
-
-
?
1.14.14.20
4-methylphenol + FADH2 + O2
Rhodococcus opacus
-
4-methylcatechol + FAD + H2O
-
-
?
1.14.14.20
additional information
Rhodococcus opacus
substrate speccificities of the three isozymes, overview
?
-
-
-
1.14.14.20
additional information
Rhodococcus opacus 1CP
substrate speccificities of the three isozymes, overview
?
-
-
-
1.14.14.20
phenol + FADH2 + O2
Rhodococcus opacus
-
catechol + FAD + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.20
2,4-dichlorophenol + FADH2 + O2
-
736121
Rhodococcus opacus
2,4-dichlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
2,4-dichlorophenol + FADH2 + O2
-
736121
Rhodococcus opacus 1CP
2,4-dichlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
2-chlorophenol + FADH2 + O2
-
736121
Rhodococcus opacus
2-chlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
2-chlorophenol + FADH2 + O2
poor substrate for isozyme PheA1(1)
736121
Rhodococcus opacus
2-chlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
2-chlorophenol + FADH2 + O2
-
736121
Rhodococcus opacus 1CP
2-chlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
2-chlorophenol + FADH2 + O2
poor substrate for isozyme PheA1(1)
736121
Rhodococcus opacus 1CP
2-chlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
4-chlorophenol + FADH2 + O2
-
736121
Rhodococcus opacus
4-chlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
4-chlorophenol + FADH2 + O2
-
736121
Rhodococcus opacus 1CP
4-chlorocatechol + FAD + H2O
-
-
-
?
1.14.14.20
4-methylphenol + FADH2 + O2
-
736121
Rhodococcus opacus
4-methylcatechol + FAD + H2O
-
-
-
?
1.14.14.20
additional information
substrate speccificities of the three isozymes, overview
736121
Rhodococcus opacus
?
-
-
-
-
1.14.14.20
additional information
substrate speccificities of the three isozymes, overview
736121
Rhodococcus opacus 1CP
?
-
-
-
-
1.14.14.20
phenol + FADH2 + O2
-
736121
Rhodococcus opacus
catechol + FAD + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.14.20
oligomer
homotetrameric or homohexameric structure, all phenol hydroxylase isoenzymes, x * 59000-63000, SDS-PAGE
Rhodococcus opacus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.14.20
25
-
assay at
Rhodococcus opacus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.14.20
7.2
-
assay at
Rhodococcus opacus
General Information
EC Number
General Information
Commentary
Organism
1.14.14.20
evolution
phylogenetic analysis shows exceptional high similarities of PheA1(1-3) and PheA2(1-3) to putative phenol hydroxylases in several Rhodococcus strains, overview
Rhodococcus opacus
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.14.20
evolution
phylogenetic analysis shows exceptional high similarities of PheA1(1-3) and PheA2(1-3) to putative phenol hydroxylases in several Rhodococcus strains, overview
Rhodococcus opacus