EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.3.13 | expressed in Escherichia coli BL21(DE3) Star cells | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.3.13 | H167A | the mutant shows reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.3.3.13 | L143A | the mutant shows reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.3.3.13 | L143N | the mutant shows reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.3.3.13 | N250A | the mutant shows reduced activity and is able to use (3RS)-3-methyl-2-oxovalerate and 4-methyl-2-oxovalerate as substrates compared to the wild type enzyme | Mycobacterium tuberculosis |
2.3.3.13 | S216G | the mutant shows reduced activity and is able to use (3RS)-3-methyl-2-oxovalerate and 4-methyl-2-oxovalerate as substrates compared to the wild type enzyme | Mycobacterium tuberculosis |
2.3.3.13 | Y169A | the mutant shows reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
2.3.3.13 | Y169H | the mutant shows reduced activity compared to the wild type enzyme | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Mycobacterium tuberculosis | - |
(2S)-2-isopropylmalate + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.13 | Mycobacterium tuberculosis | P9WQB3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.13 | HiTrap affinity column chromatography and gel Sephacryl 26/60 filtration | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | acetyl-CoA + 2-oxobutyrate + H2O | weak substrate | Mycobacterium tuberculosis | ? | - |
? | |
2.3.3.13 | acetyl-CoA + 2-oxovalerate + H2O | weak substrate | Mycobacterium tuberculosis | ? | - |
? | |
2.3.3.13 | acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Mycobacterium tuberculosis | (2S)-2-isopropylmalate + CoA | - |
? | |
2.3.3.13 | acetyl-CoA + pyruvate + H2O | weak substrate | Mycobacterium tuberculosis | ? | - |
? | |
2.3.3.13 | additional information | no activity with (3RS)-3-methyl-2-oxovalerate and 4-methyl-2-oxovalerate | Mycobacterium tuberculosis | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.3.13 | Alpha-isopropylmalate synthase | - |
Mycobacterium tuberculosis |
2.3.3.13 | IPMS | - |
Mycobacterium tuberculosis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.3.13 | metabolism | the enzyme catalyzes the first step of leucine biosynthesis | Mycobacterium tuberculosis |