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Literature summary extracted from

  • Hunter, M.F.; Parker, E.J.
    Modifying the determinants of alpha-ketoacid substrate selectivity in Mycobacterium tuberculosis alpha-isopropylmalate synthase (2014), FEBS Lett., 588, 1603-1607.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.13 expressed in Escherichia coli BL21(DE3) Star cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.3.13 H167A the mutant shows reduced activity compared to the wild type enzyme Mycobacterium tuberculosis
2.3.3.13 L143A the mutant shows reduced activity compared to the wild type enzyme Mycobacterium tuberculosis
2.3.3.13 L143N the mutant shows reduced activity compared to the wild type enzyme Mycobacterium tuberculosis
2.3.3.13 N250A the mutant shows reduced activity and is able to use (3RS)-3-methyl-2-oxovalerate and 4-methyl-2-oxovalerate as substrates compared to the wild type enzyme Mycobacterium tuberculosis
2.3.3.13 S216G the mutant shows reduced activity and is able to use (3RS)-3-methyl-2-oxovalerate and 4-methyl-2-oxovalerate as substrates compared to the wild type enzyme Mycobacterium tuberculosis
2.3.3.13 Y169A the mutant shows reduced activity compared to the wild type enzyme Mycobacterium tuberculosis
2.3.3.13 Y169H the mutant shows reduced activity compared to the wild type enzyme Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Mycobacterium tuberculosis
-
(2S)-2-isopropylmalate + CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.13 Mycobacterium tuberculosis P9WQB3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.13 HiTrap affinity column chromatography and gel Sephacryl 26/60 filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.13 acetyl-CoA + 2-oxobutyrate + H2O weak substrate Mycobacterium tuberculosis ?
-
?
2.3.3.13 acetyl-CoA + 2-oxovalerate + H2O weak substrate Mycobacterium tuberculosis ?
-
?
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Mycobacterium tuberculosis (2S)-2-isopropylmalate + CoA
-
?
2.3.3.13 acetyl-CoA + pyruvate + H2O weak substrate Mycobacterium tuberculosis ?
-
?
2.3.3.13 additional information no activity with (3RS)-3-methyl-2-oxovalerate and 4-methyl-2-oxovalerate Mycobacterium tuberculosis ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.13 Alpha-isopropylmalate synthase
-
Mycobacterium tuberculosis
2.3.3.13 IPMS
-
Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.3.3.13 metabolism the enzyme catalyzes the first step of leucine biosynthesis Mycobacterium tuberculosis