Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Xu, X.; Mathieu, C.; Boitard, S.E.; Dairou, J.; Dupret, J.M.; Agbulut, O.; Rodrigues-Lima, F.
    Skeletal muscle glycogen phosphorylase is irreversibly inhibited by mercury: molecular, cellular and kinetic aspects (2014), FEBS Lett., 588, 138-142.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.1 AMP
-
 Oryctolagus cuniculus
2.4.1.1 AMP
-
 Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 CH3Hg+ irreversible inhibition, partial reactivation of the enzyme by GSH, but not by EDTA, about 80% inhibition at 0.03 mM Mus musculus
2.4.1.1 CH3Hg+ irreversible inhibition, partial reactivation of the enzyme by GSH, but not by EDTA Oryctolagus cuniculus
2.4.1.1 Hg2+ irreversible inhibition, partial reactivation of the enzyme by GSH, but not by EDTA, about 90% inhibition at 0.0125 mM Hg2+, 80% at 0.01 mM Mus musculus
2.4.1.1 Hg2+ irreversible inhibition, partial reactivation of the enzyme by GSH, but not by EDTA Oryctolagus cuniculus
2.4.1.1 additional information molecular and kinetic mechanisms of enzyme inhibition by mercury, overview Mus musculus
2.4.1.1 additional information molecular and kinetic mechanisms of enzyme inhibition by mercury, overview Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate Oryctolagus cuniculus
-
 [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate Mus musculus
-
 [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate Mus musculus Swiss
-
 [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Mus musculus Q9WUB3
-
-
2.4.1.1 Mus musculus Swiss Q9WUB3
-
-
2.4.1.1 Oryctolagus cuniculus P00489
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 C2C12 cell
-
 Mus musculus
-
2.4.1.1 myoblast
-
 Mus musculus
-
2.4.1.1 myotube
-
 Mus musculus
-
2.4.1.1 skeletal muscle
-
 Oryctolagus cuniculus
-
2.4.1.1 skeletal muscle
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Oryctolagus cuniculus [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Mus musculus [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate
-
 Mus musculus Swiss [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.1 muscle glycogen phosphorylase
-
 Oryctolagus cuniculus
2.4.1.1 muscle glycogen phosphorylase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.1 25
-
 assay at, glycogen synthesis Oryctolagus cuniculus
2.4.1.1 37
-
 assay at, glycogen synthesis Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.1 6.8
-
 assay at, glycogen synthesis Oryctolagus cuniculus
2.4.1.1 6.8
-
 assay at, glycogen synthesis Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.1 0.00038
-
 Hg2+ pH 6.8, 25°C Oryctolagus cuniculus
2.4.1.1 0.043
-
 CH3Hg+ pH 6.8, 25°C Oryctolagus cuniculus

General Information

EC Number General Information Comment Organism
2.4.1.1 malfunction mutations that impair glycogen phosphorylase activity leads to McArdle's disease which is characterized by exercise intolerance, muscle fatigue, and cramps. The irreversible inhibition of the enzyme might represent one of the mechanisms that contribute to mercury-dependent muscle toxicity Oryctolagus cuniculus
2.4.1.1 malfunction mutations that impair glycogen phosphorylase activity leads to McArdle's disease which is characterized by exercise intolerance, muscle fatigue, and cramps. The irreversible inhibition of the enzyme might represent one of the mechanisms that contribute to mercury-dependent muscle toxicity Mus musculus
2.4.1.1 metabolism muscle glycogen phosphorylase is the rate-limiting enzyme of glycogen breakdown in skeletal muscle Oryctolagus cuniculus
2.4.1.1 metabolism muscle glycogen phosphorylase is the rate-limiting enzyme of glycogen breakdown in skeletal muscle Mus musculus