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Literature summary extracted from

  • Figueroa, C.; Asencion Diez, M.; Kuhn, M.; McEwen, S.; Salerno, G.; Iglesias, A.; Ballicora, M.
    The unique nucleotide specificity of the sucrose synthase from Thermosynechococcus elongatus (2013), FEBS Lett., 587, 165-169.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.13 the gene is artificially synthesized for optimal recombinant expression in Escherichia coli strain Tuner (DE3) Thermosynechococcus vestitus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.13 0.033
-
ADP-glucose pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
2.4.1.13 0.18
-
ADP pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
2.4.1.13 0.9
-
CDP pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
2.4.1.13 1.1
-
GDP pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
2.4.1.13 1.3
-
UDP pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
2.4.1.13 1.4
-
TDP pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
2.4.1.13 1.7
-
UDP-glucose pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
2.4.1.13 5.6
-
D-fructose pH 7.0, 37°C, recombinant enzyme, with ADP-glucose Thermosynechococcus vestitus
2.4.1.13 12
-
D-fructose pH 7.0, 37°C, recombinant enzyme, with UDP-glucose Thermosynechococcus vestitus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.13 Mg2+ required Thermosynechococcus vestitus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.13 95000
-
4 * 95000, recombinant enzyme, SDS-PAGE Thermosynechococcus vestitus
2.4.1.13 341000
-
recombinant enzyme, gel filtration Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.13 NDP-glucose + D-fructose Thermosynechococcus vestitus
-
NDP + sucrose
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.13 Thermosynechococcus vestitus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.13 ADP-glucose + D-fructose
-
Thermosynechococcus vestitus ADP + sucrose ADP is the best acceptor substrate in sucrose cleavage r
2.4.1.13 CDP-glucose + D-fructose
-
Thermosynechococcus vestitus CDP + sucrose
-
r
2.4.1.13 GDP-glucose + D-fructose
-
Thermosynechococcus vestitus GDP + sucrose
-
r
2.4.1.13 additional information unique nucleotide specificity of the sucrose synthase from Thermosynechococcus elongatus Thermosynechococcus vestitus ?
-
?
2.4.1.13 NDP-glucose + D-fructose
-
Thermosynechococcus vestitus NDP + sucrose
-
r
2.4.1.13 TDP-glucose + D-fructose lower activity in sucrose cleavage Thermosynechococcus vestitus TDP + sucrose
-
r
2.4.1.13 UDP-glucose + D-fructose lower activity in sucrose cleavage Thermosynechococcus vestitus UDP + sucrose
-
r

Subunits

EC Number Subunits Comment Organism
2.4.1.13 homotetramer 4 * 95000, recombinant enzyme, SDS-PAGE Thermosynechococcus vestitus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.13 60
-
sucrose cleavage with UDP Thermosynechococcus vestitus
2.4.1.13 70
-
sucrose cleavage with ADP Thermosynechococcus vestitus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.13 30 75 activity range, profile overview Thermosynechococcus vestitus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.13 50
-
the enzyme remains stable after 10 min incubation up to 50°C, but activity sharply decays beyond 55°C. Addition of 1 mM ADP, 10 mM UDP, or 200 mM Suc enhances the thermal stability by almost 5°C Thermosynechococcus vestitus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.13 6
-
sucrose pH 7.0, 37°C, purified recombinant enzyme, with ADP in sucrose cleavage Thermosynechococcus vestitus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.13 7
-
sucrose cleavage with CDP, GDP, TDP, and UDP Thermosynechococcus vestitus
2.4.1.13 8
-
sucrose cleavage with ADP Thermosynechococcus vestitus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.13 7 9 sucrose cleavage, activities with CDP, GDP, TDP, and UDP only occur at pH 7.0-8.0 and are very low at pH 9.0, while the activity with ADP is highest at pH 8.0 and higher than 60% of maximal activity in the pH range of 7.0-9.0 Thermosynechococcus vestitus

General Information

EC Number General Information Comment Organism
2.4.1.13 metabolism interaction between sucrose and glycogen metabolism Thermosynechococcus vestitus
2.4.1.13 physiological function in filamentous cyanobacteria, the sucrose cleavage direction plays a key physiological function in carbon metabolism, nitrogen fixation, and stress tolerance Thermosynechococcus vestitus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.13 0.38
-
D-fructose pH 7.0, 37°C, recombinant enzyme, with UDP-glucose Thermosynechococcus vestitus
2.4.1.13 0.406
-
D-fructose pH 7.0, 37°C, recombinant enzyme, with ADP-glucose Thermosynechococcus vestitus
2.4.1.13 2.673
-
UDP-glucose pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus
2.4.1.13 70.09
-
ADP-glucose pH 7.0, 37°C, recombinant enzyme Thermosynechococcus vestitus