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Literature summary extracted from

  • Addlesee, H.A.; Gibson, L.C.; Jensen, P.E.; Hunter, C.N.
    Cloning, sequencing and functional assignment of the chlorophyll biosynthesis gene, chlP, of Synechocystis sp. PCC 6803 (1996), FEBS Lett., 389, 126-130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.111 gene bchP, sequence comparison with gene chlP from Synechocystis sp. strain PCC 6803 Cereibacter sphaeroides
1.3.1.111 gene chfP, DNA and amino acid sequence determination and analysis, the enzyme partially complements a bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides, which is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex. Upon heterologous expression of the Synechocystis bchP homologue, not only are hydrogenated forms of geranylgeranyl bacteriochlorophyllide a (bchlaGG) detectable, but the level of LH2 is increased, sequence comparison with gene chP from Rhodobacter sphaeroides strain NCIB 8253 Synechocystis sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.111 additional information the bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex. Upon heterologous expression of the Synechocystis bchP homologue encoded by gene chlP, not only are hydrogenated forms of geranylgeranyl bacteriochlorophyllide a (bchlaGG) detectable, but the level of LH2 is increased, phenotype, overview Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.111 44869
-
 x * 44869, sequence calculation Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.111 bacteriochlorophyll a + 3 NADP+ Cereibacter sphaeroides
-
 geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?
1.3.1.111 bacteriochlorophyll a + 3 NADP+ Synechocystis sp.
-
 geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?
1.3.1.111 bacteriochlorophyll a + 3 NADP+ Cereibacter sphaeroides NCIB 8253
-
 geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.111 Cereibacter sphaeroides Q9Z5D4 gene bchP
-
1.3.1.111 Cereibacter sphaeroides NCIB 8253 Q9Z5D4 gene bchP
-
1.3.1.111 Synechocystis sp. Q55087 gene chlP; gene chlP
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.111 bacteriochlorophyll a + 3 NADP+
-
 Cereibacter sphaeroides geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?
1.3.1.111 bacteriochlorophyll a + 3 NADP+
-
 Synechocystis sp. geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?
1.3.1.111 bacteriochlorophyll a + 3 NADP+
-
 Cereibacter sphaeroides NCIB 8253 geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.111 ? x * 44869, sequence calculation Synechocystis sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.111 NADP+
-
 Cereibacter sphaeroides
1.3.1.111 NADP+
-
 Synechocystis sp.

General Information

EC Number General Information Comment Organism
1.3.1.111 malfunction the bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex Cereibacter sphaeroides
1.3.1.111 metabolism the enzyme catalyzes the stepwise hydrogenation of geranylgeraniol to phytol during bacteriochlorophyll a biosynthesis Synechocystis sp.
1.3.1.111 physiological function the enzyme catalyzes the stepwise hydrogenation of geranylgeraniol to phytol during bacteriochlorophyll a biosynthesis Synechocystis sp.