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Literature summary extracted from

  • Haddad Momeni, M.; Ubhayasekera, W.; Sandgren, M.; Stahlberg, J.; Hansson, H.
    Structural insights into the inhibition of cellobiohydrolase Cel7A by xylo-oligosaccharides (2015), FEBS J., 282, 2167-2177.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.176 structures in complex with xylotriose, xylotetraose and xylopentaose reveal a predominant binding mode at the entrance of the substrate-binding tunnel of the enzyme, in which each xylose residue is shifted about 2.4 A towards the catalytic center compared with binding of cellooligosaccharides. Partial occupancy of two consecutive xylose residues at subsites -2 and -1 suggests an alternative binding mode for xylooligosaccharides in the vicinity of the catalytic center Trichoderma reesei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.176 birchwood xylan mixed inhibition Trichoderma reesei
3.2.1.176 additional information xylose, Ki value above 1 mM Trichoderma reesei
3.2.1.176 xylobiose mixed inhibition Trichoderma reesei
3.2.1.176 xylopentaose mixed inhibition Trichoderma reesei
3.2.1.176 xylotriose mixed inhibition Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.176 0.0016
-
4-nitrophenyl lactoside pH 5.0, 37°C Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Trichoderma reesei G0RVK1
-
-
3.2.1.176 Trichoderma reesei QM6a G0RVK1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 4-nitrophenyl lactoside + H2O
-
Trichoderma reesei 4-nitrophenol + lactose
-
?
3.2.1.176 4-nitrophenyl lactoside + H2O
-
Trichoderma reesei QM6a 4-nitrophenol + lactose
-
?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.176 additional information
-
birchwood xylan Kic 0.093 g/l, Kiu 0.18 g/l, pH 5.0, 37°C Trichoderma reesei
3.2.1.176 0.0097
-
xylobiose Kic, pH 5.0, 37°C Trichoderma reesei
3.2.1.176 0.014
-
xylopentaose Kic, pH 5.0, 37°C Trichoderma reesei
3.2.1.176 0.026
-
xylopentaose Kiu, pH 5.0, 37°C Trichoderma reesei
3.2.1.176 0.029
-
xylotriose Kic, pH 5.0, 37°C Trichoderma reesei
3.2.1.176 0.056
-
xylotriose Kiu, pH 5.0, 37°C Trichoderma reesei
3.2.1.176 0.067
-
xylobiose Kiu, pH 5.0, 37°C Trichoderma reesei