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Literature summary extracted from

  • Siiskonen, H.; Kaernae, R.; Hyttinen, J.M.; Tammi, R.H.; Tammi, M.I.; Rilla, K.
    Hyaluronan synthase 1 (HAS1) produces a cytokine-and glucose-inducible, CD44-dependent cell surface coat (2014), Exp. Cell Res., 320, 153-163.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.212 gene HAS1, recombinant expression of GFP-tagged isozyme HAS1 in MCF-7 cells, MCF-7 cells transfected with Dendra-Has1 show intense fluorescence in perinuclear vesicular structures resembling the Golgi apparatus. The MCF-7 cells transfected with EGFP-Has1 produce a pericellular hyaluronan coat, which is attached the synthase itself Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.212 additional information HAS1-transfected MCF-7cells show very little cell surface hyaluronan, but a large hyaluronan coat is seen in cells grown in 20 mM glucose and 1 mM glucosamine, or treated with interleukin-1beta, TNF-alpha, or TGF-beta. The coats are mostly removed by the presence of hyaluronan hexasaccharides, or Hermes1 antibody, indicating that they depend on the CD44 receptor, which is, in contrast to the coat produced by HAS3, remaining attached to HAS3 itself Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.212 4-Methylumbelliferone
-
 Homo sapiens
2.4.1.212 brefeldin A
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.212 cell surface
-
 Homo sapiens 9986
-
2.4.1.212 Golgi apparatus
-
 Homo sapiens 5794
-
2.4.1.212 additional information the HAS1 enzymatic activity depends on the endoplasmic reticulum-Golgi-plasma membrane traffic, like reported for isozymes HAS2 and HAS3 Homo sapiens
-
-
2.4.1.212 plasma membrane
-
 Homo sapiens 5886
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.212 Homo sapiens
-
 gene HAS1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.212 breast cancer cell
-
 Homo sapiens
-
2.4.1.212 epidermis
-
 Homo sapiens
-
2.4.1.212 HaCaT cell
-
 Homo sapiens
-
2.4.1.212 keratinocyte
-
 Homo sapiens
-
2.4.1.212 MCF-7 cell very low expression of HAS1 Homo sapiens
-
2.4.1.212 additional information subcellular localization study, intracellular localization of EGFP-HAS1 and Dendra-HAS1 in MCF-7 cells, overview Homo sapiens
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.212 HAS1
-
 Homo sapiens
2.4.1.212 hyaluronan synthase 1
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
2.4.1.212 Homo sapiens a cytokine-and glucose-inducible enzyme up

General Information

EC Number General Information Comment Organism
2.4.1.212 physiological function hyaluronan is a ubiquitous glycosaminoglycan involved in embryonic development, inflammation and cancer. In mammals, three hyaluronan synthase isoenzymes (HAS1-3) inserted in the plasma membrane produce hyaluronan directly on the cell surface. Isozyme hyaluronan synthase 1 (HAS1) produces a cytokine-and glucose-inducible, CD44-dependent cell surface coat Homo sapiens