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Literature summary extracted from

  • Kowolik, C.; Hengstenberg, W.
    The lactose transporter of Staphylococcus aureus - Overexpression, purification and characterization of the histidine-tagged domains IIC and IIB (1998), Eur. J. Biochem., 257, 389-394.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.207 domain IIC fused with a C-terminal affinity tag of six histidine residues and domain IIB fused with a N-terminal histidine tag are expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.207 C476S inactive IICBlac mutant Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.207 2-(N-dansyl)aminoethyl alpha-D-thiogalactoside
-
 Staphylococcus aureus
2.7.1.207 isopropyl thio-beta-D-galactoside
-
 Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.207 membrane
-
 Staphylococcus aureus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.207 [protein]-Npi-phospho-L-histidine + lactose[side 1] Staphylococcus aureus
-
 [protein]-L-histidine + lactose 6'-phosphate[side 2]
-
 ?
2.7.1.207 [protein]-Npi-phospho-L-histidine + lactose[side 1] Staphylococcus aureus 825A
-
 [protein]-L-histidine + lactose 6'-phosphate[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.207 Staphylococcus aureus
-
-
-
2.7.1.207 Staphylococcus aureus 825A
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.207 Ni21 chelate affinity column chromatography Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.207 0.0008
-
 unpurified His-tagged recombinant domain IIClac, at pH 7.6 and 37°C Staphylococcus aureus
2.7.1.207 0.0433
-
 54.1fold purified His-tagged recombinant domain IIClac, at pH 7.6 and 37°C Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.207 [protein]-Npi-phospho-L-histidine + lactose[side 1]
-
 Staphylococcus aureus [protein]-L-histidine + lactose 6'-phosphate[side 2]
-
 ?
2.7.1.207 [protein]-Npi-phospho-L-histidine + lactose[side 1]
-
 Staphylococcus aureus 825A [protein]-L-histidine + lactose 6'-phosphate[side 2]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.207 IICBlac
-
 Staphylococcus aureus
2.7.1.207 lactose transporter
-
 Staphylococcus aureus
2.7.1.207 lactose-specific enzyme II
-
 Staphylococcus aureus