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Literature summary extracted from

  • Lopez, G.; Quezada, H.; Duhne, M.; Gonzalez, J.; Lezama, M.; El-Hafidi, M.; Colon, M.; Martinez de la Escalera, X.; Flores-Villegas, M.C.; Scazzocchio, C.; DeLuna, A.; Gonzalez, A.
    Diversification of paralogous alpha-isopropylmalate synthases by modulation of feedback control and hetero-oligomerization in Saccharomyces cerevisiae (2015), Eukaryot. Cell, 14, 564-577.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.13 L-leucine isoform Leu4, but not Leu9, is feedback inhibited by L-leucine Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.13 mitochondrion
-
Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.13 60000
-
2 * 60000, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Saccharomyces cerevisiae
-
(2S)-2-isopropylmalate + CoA
-
?
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Saccharomyces cerevisiae BY4741
-
(2S)-2-isopropylmalate + CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.13 Saccharomyces cerevisiae
-
-
-
2.3.3.13 Saccharomyces cerevisiae BY4741
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.13 Q sepharose column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Saccharomyces cerevisiae (2S)-2-isopropylmalate + CoA
-
?
2.3.3.13 acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Saccharomyces cerevisiae BY4741 (2S)-2-isopropylmalate + CoA
-
?

Subunits

EC Number Subunits Comment Organism
2.3.3.13 homodimer 2 * 60000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.3.3.13 alpha-IPM synthase
-
Saccharomyces cerevisiae
2.3.3.13 alpha-IPMS
-
Saccharomyces cerevisiae
2.3.3.13 Alpha-isopropylmalate synthase
-
Saccharomyces cerevisiae
2.3.3.13 LEU4 isoform Saccharomyces cerevisiae
2.3.3.13 LEU9 isoform Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.13 7.28
-
3-methyl-2-oxobutanoate isoform Leu9, at pH 7.5 and 30°C Saccharomyces cerevisiae
2.3.3.13 13.79
-
3-methyl-2-oxobutanoate isoform Leu4, at pH 7.5 and 30°C Saccharomyces cerevisiae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.3.13 0.03
-
at pH 7.5 and 30°C Saccharomyces cerevisiae L-leucine