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Literature summary extracted from

  • Donaldson, T.M.; Cassera, M.B.; Ho, M.C.; Zhan, C.; Merino, E.F.; Evans, G.B.; Tyler, P.C.; Almo, S.C.; Schramm, V.L.; Kim, K.
    Inhibition and structure of Toxoplasma gondii purine nucleoside phosphorylase (2014), Eukaryot. Cell, 13, 572-579.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.1 expressed in Escherichia coli BL21-Codon plus cells Plasmodium falciparum
2.4.2.1 expressed in Escherichia coli BL21-Codon plus cells Toxoplasma gondii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.1 in complex with phosphate and immucillin-H, sitting drop vapor diffusion method, using 25% (w/v) polyethylene glycol monomethyl ether 2000, 100 mM Tris (pH 8.5), and 0.2 M trimethylamine N-oxide dihydrate Plasmodium falciparum
2.4.2.1 in complex with phosphate and immucillin-H, sitting drop vapor diffusion method, using 25% (w/v) polyethylene glycol monomethyl ether 2000, 100 mM Tris (pH 8.5), and 0.2 M trimethylamine N-oxide dihydrate Toxoplasma gondii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 1',9-methyl-immucillin-H
-
 Plasmodium falciparum
2.4.2.1 1',9-methyl-immucillin-H
-
 Toxoplasma gondii
2.4.2.1 2'-deoxy-immucillin-H
-
 Plasmodium falciparum
2.4.2.1 2'-deoxy-immucillin-H
-
 Toxoplasma gondii
2.4.2.1 4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-G
-
 Plasmodium falciparum
2.4.2.1 4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-G
-
 Toxoplasma gondii
2.4.2.1 4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-H
-
 Plasmodium falciparum
2.4.2.1 4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-H
-
 Toxoplasma gondii
2.4.2.1 5'-amido-immucillin-H
-
 Plasmodium falciparum
2.4.2.1 5'-amido-immucillin-H
-
 Toxoplasma gondii
2.4.2.1 5'-carboxy-immucillin-H
-
 Plasmodium falciparum
2.4.2.1 5'-carboxy-immucillin-H
-
 Toxoplasma gondii
2.4.2.1 5'-deoxy-immucillin-H
-
 Plasmodium falciparum
2.4.2.1 5'-deoxy-immucillin-H
-
 Toxoplasma gondii
2.4.2.1 5'-fluoro-immucillin-H
-
 Plasmodium falciparum
2.4.2.1 5'-fluoro-immucillin-H
-
 Toxoplasma gondii
2.4.2.1 5'-methylthio-immucillin-H
-
 Plasmodium falciparum
2.4.2.1 5'-methylthio-immucillin-H
-
 Toxoplasma gondii
2.4.2.1 5'-thio-immucillin-H
-
 Plasmodium falciparum
2.4.2.1 5'-thio-immucillin-H
-
 Toxoplasma gondii
2.4.2.1 immucillin-H transition-state analogue inhibitor Plasmodium falciparum
2.4.2.1 immucillin-H transition-state analogue inhibitor Toxoplasma gondii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.004
-
 Inosine pH 7.4, temperature not specified in the publication Toxoplasma gondii
2.4.2.1 0.011
-
 Inosine pH 7.4, temperature not specified in the publication Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Plasmodium falciparum
-
-
-
2.4.2.1 Toxoplasma gondii Q2HXR2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1 Ni-NTA affinity column chromatography, and gel filtration Plasmodium falciparum
2.4.2.1 Ni-NTA affinity column chromatography, and gel filtration Toxoplasma gondii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.1 tachyzoite
-
 Plasmodium falciparum
-
2.4.2.1 tachyzoite
-
 Toxoplasma gondii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 inosine + phosphate
-
 Plasmodium falciparum hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Toxoplasma gondii hypoxanthine + alpha-D-ribose 1-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.2.1 PNP
-
 Plasmodium falciparum
2.4.2.1 PNP
-
 Toxoplasma gondii
2.4.2.1 purine nucleoside phosphorylase
-
 Plasmodium falciparum
2.4.2.1 purine nucleoside phosphorylase
-
 Toxoplasma gondii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 0.23
-
 Inosine pH 7.4, temperature not specified in the publication Toxoplasma gondii
2.4.2.1 1.7
-
 Inosine pH 7.4, temperature not specified in the publication Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.1 0.000000093
-
 5'-thio-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
2.4.2.1 0.00000027
-
 5'-methylthio-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
2.4.2.1 0.00000037
-
 immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
2.4.2.1 0.0000005
-
 4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
2.4.2.1 0.00000086
-
 immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
2.4.2.1 0.0000009
-
 4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-G pH 7.4, temperature not specified in the publication Plasmodium falciparum
2.4.2.1 0.0000011
-
 5'-deoxy-immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
2.4.2.1 0.0000016
-
 5'-deoxy-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
2.4.2.1 0.0000019
-
 2'-deoxy-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
2.4.2.1 0.000002
-
 5'-fluoro-immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
2.4.2.1 0.0000022
-
 5'-fluoro-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
2.4.2.1 0.000012
-
 5'-carboxy-immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
2.4.2.1 0.000017
-
 5'-carboxy-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
2.4.2.1 0.000019
-
 2'-deoxy-immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
2.4.2.1 0.00022
-
 5'-amido-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
2.4.2.1 0.0015
-
 4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-G pH 7.4, temperature not specified in the publication Toxoplasma gondii
2.4.2.1 0.0036
-
 4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
2.4.2.1 0.0056
-
 5'-methylthio-immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
2.4.2.1 0.0089
-
 5'-amido-immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
2.4.2.1 0.05
-
 5'-thio-immucillin-H Ki above 0.05 mM, pH 7.4, temperature not specified in the publication Toxoplasma gondii
2.4.2.1 0.05
-
 1',9-methyl-immucillin-H Ki above 0.05 mM, pH 7.4, temperature not specified in the publication Toxoplasma gondii
2.4.2.1 0.6
-
 1',9-methyl-immucillin-H Ki above 0.6 mM, pH 7.4, temperature not specified in the publication Plasmodium falciparum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.2.1 15
-
 Inosine pH 7.4, temperature not specified in the publication Plasmodium falciparum
2.4.2.1 55
-
 Inosine pH 7.4, temperature not specified in the publication Toxoplasma gondii