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Literature summary extracted from

  • Xin, D.; Ge, X.; Sun, Z.; Viikari, L.; Zhang, J.
    Competitive inhibition of cellobiohydrolase I by manno-oligosaccharides (2015), Enzyme Microb. Technol., 68, 62-68.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.176 degradation commercial cellulases contain mannan hydrolysing enzymes. Addition of 10 mg/ml mannan reduces the glucose yield of avicel (at 20 g/l) from 40.1 to 24.3%. The inhibitory effect is at least partly attributed to the inhibition of Cel7A(CBHI), but not on beta-glucosidase Thermoascus aurantiacus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.176 mannobiose competitive Thermoascus aurantiacus
3.2.1.176 Mannotetraose competitive Thermoascus aurantiacus
3.2.1.176 Mannotriose competitive Thermoascus aurantiacus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.176 0.67
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 50°C Thermoascus aurantiacus
3.2.1.176 0.76
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 50°C, presence of 1 mM mannotetraose Thermoascus aurantiacus
3.2.1.176 0.81
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 50°C, presence of 1 mM mannotriose Thermoascus aurantiacus
3.2.1.176 0.87
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 50°C, presence of 1 mM mannobiose Thermoascus aurantiacus
3.2.1.176 1.02
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 50°C, presence of 2 mM mannobiose Thermoascus aurantiacus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.176 Thermoascus aurantiacus A7WNU2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.176 4-nitrophenyl beta-D-cellobioside + H2O
-
 Thermoascus aurantiacus 4-nitrophenol + D-cellobiose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.176 CBHI
-
 Thermoascus aurantiacus
3.2.1.176 Cel7A
-
 Thermoascus aurantiacus