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Literature summary extracted from

  • Chen, Y.J.; Tsai, P.C.; Hsu, C.H.; Lee, C.Y.
    Critical residues of class II PHA synthase for expanding the substrate specificity and enhancing the biosynthesis of polyhydroxyalkanoate (2014), Enzyme Microb. Technol., 56, 60-66.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.304 synthesis expansion of the substrate specificity and enhancment of biosynthesis of polyhydroxyalkanoate by site-specific mutagenesis. Mutated PhaC1s are coexpressed with beta-ketothiolase and acetoacetyl-CoA reductase to supply sufficient short-chain length (R)-3-hydroxyacyl-CoA as a substrate. Mutation L484V remarkably enhances the monomer ratio of (R)-3-hydroxybutyrate in a polyhydroxyalkanoate accumulation experiment. Val is the most favorable amino acid for incorporating (R)-3-hydroxybutyrate unit synthesis. A single mutation at Q481M, S482G and A547V obviously increases polyhydroxyalkanoate yields. Q481M and S482G enhance the (R)-3-hydroxyhexanoate monomer composition in the polyhydroxyalkanoate accumulation Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.304 expression in Pseudomonas putida GPp104 PHA mutant Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.304 A547V mutation increases polyhydroxyalkanoate yields Pseudomonas putida
2.3.1.304 L484V mutation remarkably enhances the monomer ratio of (R)-3-hydroxybutyrate in a polyhydroxyalkanoate accumulation experiment. Val is the most favorable amino acid for incorporating (R)-3-hydroxybutyrate unit synthesis Pseudomonas putida
2.3.1.304 Q481M mutation increases polyhydroxyalkanoate yields and enhances the (R)-3-hydroxyhexanoate monomer composition in the polyhydroxyalkanoate accumulation Pseudomonas putida
2.3.1.304 S482G mutation increases polyhydroxyalkanoate yields and enhances the (R)-3-hydroxyhexanoate monomer composition in the polyhydroxyalkanoate accumulation Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Pseudomonas putida
-
-
-
2.3.1.304 Pseudomonas putida GPo1 / ATCC29347
-
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.304 PhaC1
-
 Pseudomonas putida