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Literature summary extracted from

  • Jeong, Y.J.; An, C.H.; Woo, S.G.; Jeong, H.J.; Kim, Y.M.; Park, S.J.; Yoon, B.D.; Kim, C.Y.
    Production of pinostilbene compounds by the expression of resveratrol O-methyltransferase genes in Escherichia coli (2014), Enzyme Microb. Technol., 54, 8-14.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.240 gene SbROMT3syn, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, subcloning in Escherichia coli strain DH5alpha Sorghum bicolor
2.1.1.240 gene VrROMTsyn, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, subcloning in Escherichia coli strain DH5alpha. Recombinantly expressed enzyme from grape produces very small amounts of methylated resveratrol derivatives in Escherichia coli Vitis riparia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.240 Mg2+ required Sorghum bicolor
2.1.1.240 Mg2+ required Vitis riparia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.240 S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene Sorghum bicolor i.e. pinostilbene S-adenosyl-L-homocysteine + pterostilbene
-
 ?
2.1.1.240 S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene Vitis riparia i.e. pinostilbene S-adenosyl-L-homocysteine + pterostilbene
-
 ?
2.1.1.240 S-adenosyl-L-methionine + trans-resveratrol Sorghum bicolor
-
 S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
-
 ?
2.1.1.240 S-adenosyl-L-methionine + trans-resveratrol Vitis riparia
-
 S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.240 Sorghum bicolor A8QW53 gene SbROMT3syn
-
2.1.1.240 Vitis riparia K7XQ68 gene VrROMTsyn
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.240 S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene i.e. pinostilbene Sorghum bicolor S-adenosyl-L-homocysteine + pterostilbene
-
 ?
2.1.1.240 S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene i.e. pinostilbene Vitis riparia S-adenosyl-L-homocysteine + pterostilbene
-
 ?
2.1.1.240 S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene i.e. pinostilbene, minor reaction, recombinant SbROMT3syn produces pinostilbene as the major product besides a very small amount of pterostilbene Sorghum bicolor S-adenosyl-L-homocysteine + pterostilbene
-
 ?
2.1.1.240 S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene i.e. pinostilbene, minor reaction, recombinant SbROMT3syn produces pinostilbene as the major product besides a very small amount of pterostilbene Vitis riparia S-adenosyl-L-homocysteine + pterostilbene
-
 ?
2.1.1.240 S-adenosyl-L-methionine + trans-resveratrol
-
 Sorghum bicolor S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
-
 ?
2.1.1.240 S-adenosyl-L-methionine + trans-resveratrol
-
 Vitis riparia S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
-
 ?
2.1.1.240 S-adenosyl-L-methionine + trans-resveratrol major reaction step, recombinant SbROMT3syn produces pinostilbene as the major product besides a very small amount of pterostilbene Sorghum bicolor S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
-
 ?
2.1.1.240 S-adenosyl-L-methionine + trans-resveratrol major reaction step, recombinant SbROMT3syn produces pinostilbene as the major product besides a very small amount of pterostilbene Vitis riparia S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.240 resveratrol O-methyltransferase
-
 Sorghum bicolor
2.1.1.240 resveratrol O-methyltransferase
-
 Vitis riparia
2.1.1.240 ROMT
-
 Sorghum bicolor
2.1.1.240 ROMT
-
 Vitis riparia
2.1.1.240 SbROMT3syn protein
-
 Sorghum bicolor
2.1.1.240 VrROMTsyn protein
-
 Vitis riparia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.240 30
-
 assay at Sorghum bicolor
2.1.1.240 30
-
 assay at Vitis riparia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.240 7.5
-
 assay at Sorghum bicolor
2.1.1.240 7.5
-
 assay at Vitis riparia

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.240 S-adenosyl-L-methionine
-
 Sorghum bicolor
2.1.1.240 S-adenosyl-L-methionine
-
 Vitis riparia

General Information

EC Number General Information Comment Organism
2.1.1.240 metabolism biosynthetic pathway of stilbene compound production from phenylalanine involving the enzyme, overview Sorghum bicolor
2.1.1.240 metabolism biosynthetic pathway of stilbene compound production from phenylalanine involving the enzyme, overview Vitis riparia