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Literature summary extracted from

  • Hyvoenen, M.; Weisell, J.; Khomutov, A.; Alhonen, L.; Vepsaelaeinen, J.; Keinaenen, T.
    Metabolism of triethylenetetramine and 1,12-diamino-3,6,9-triazadodecane by the spermidine/spermine-N1-acetyltransferase and thialysine acetyltransferase (2013), Drug Metab. Dispos., 41, 30-32.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.57 additional information knockdown of enzyme SSAT1 in Hep-G2 cells and in primary hepatocytes Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.57 0.083
-
 triethylenetetramine pH and temperature not specified in the publication Homo sapiens
2.3.1.57 0.106
-
 1,12-diamino-3,6,9-triazadodecane pH and temperature not specified in the publication Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.57 3 acetyl-CoA + 2 spermine Homo sapiens
-
 3 CoA + N1-acetylspermine + N1,N12-diacetylspermine
-
 ?
2.3.1.57 3 acetyl-CoA + 2 spermine Mus musculus
-
 3 CoA + N1-acetylspermine + N1,N12-diacetylspermine
-
 ?
2.3.1.57 3 acetyl-CoA + spermidine Homo sapiens
-
 3 CoA + N1,N4,N8-triacetylspermidine
-
 ?
2.3.1.57 3 acetyl-CoA + spermidine Mus musculus
-
 3 CoA + N1,N4,N8-triacetylspermidine
-
 ?
2.3.1.57 acetyl-CoA + 1,12-diamino-3,6,9-triazadodecane Homo sapiens an extremely poor substrate of human recombinant SSAT2, that is metabolized by SSAT1 in Hep-G2 cells and in wild-type primary hepatocytes ?
-
 ?
2.3.1.57 acetyl-CoA + 1,12-diamino-3,6,9-triazadodecane Mus musculus SSAT1 is the main acetylator of 1,12-diamino-3,6,9-triazadodecane compared to SSAT2 ?
-
 ?
2.3.1.57 acetyl-CoA + triethylenetetramine Homo sapiens SSAT2 is the main acetylator of TETA compared to SSAT1 ?
-
 ?
2.3.1.57 acetyl-CoA + triethylenetetramine Mus musculus SSAT2 is the main acetylator of TETA compared to SSAT1 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.57 Homo sapiens P21673
-
-
2.3.1.57 Mus musculus P48026
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.57 Hep-G2 cell
-
 Homo sapiens
-
2.3.1.57 hepatocyte primary Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.57 3 acetyl-CoA + 2 spermine
-
 Homo sapiens 3 CoA + N1-acetylspermine + N1,N12-diacetylspermine
-
 ?
2.3.1.57 3 acetyl-CoA + 2 spermine
-
 Mus musculus 3 CoA + N1-acetylspermine + N1,N12-diacetylspermine
-
 ?
2.3.1.57 3 acetyl-CoA + spermidine
-
 Homo sapiens 3 CoA + N1,N4,N8-triacetylspermidine
-
 ?
2.3.1.57 3 acetyl-CoA + spermidine
-
 Mus musculus 3 CoA + N1,N4,N8-triacetylspermidine
-
 ?
2.3.1.57 acetyl-CoA + 1,12-diamino-3,6,9-triazadodecane an extremely poor substrate of human recombinant SSAT2, that is metabolized by SSAT1 in Hep-G2 cells and in wild-type primary hepatocytes Homo sapiens ?
-
 ?
2.3.1.57 acetyl-CoA + 1,12-diamino-3,6,9-triazadodecane SSAT1 is the main acetylator of 1,12-diamino-3,6,9-triazadodecane compared to SSAT2 Homo sapiens ?
-
 ?
2.3.1.57 acetyl-CoA + 1,12-diamino-3,6,9-triazadodecane SSAT1 is the main acetylator of 1,12-diamino-3,6,9-triazadodecane compared to SSAT2 Mus musculus ?
-
 ?
2.3.1.57 acetyl-CoA + triethylenetetramine SSAT2 is the main acetylator of TETA compared to SSAT1 Homo sapiens ?
-
 ?
2.3.1.57 acetyl-CoA + triethylenetetramine SSAT2 is the main acetylator of TETA compared to SSAT1 Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.57 spermidine/spermine-N1-acetyltransferase
-
 Homo sapiens
2.3.1.57 spermidine/spermine-N1-acetyltransferase
-
 Mus musculus
2.3.1.57 SSAT1
-
 Homo sapiens
2.3.1.57 SSAT1
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.57 acetyl-CoA
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.57 malfunction acetylation of triethylenetetramine is increased in SSAT1-overexpressing mice compared with wild-type mice, but SSAT1-deficient mice metabolize TETA at the same rate as the wild-type mice, due to the activity of thialysine acetyltransferase (SSAT2) Mus musculus
2.3.1.57 malfunction SSAT1 knockdown leads to a dramatic reduction of N1-acetyylspermidine and N1-acetylspermine pools. Metabolism of 1,12-diamino-3,6,9-triazadodecane to N1-acetyl-1,12-diamino-3,6,9-triazadodecane is reduced with SSAT1 but not with SSAT2 shRNA. No metabolism of 1,12-diamino-3,6,9-triazadodecane detectable in SSAT1-KO cells. In wild-type cells, SSAT2 knockdown does not reduce the metabolism of 1,12-diamino-3,6,9-triazadodecane to N1-AcSpmTrien. In fact it leads to the induction of SSAT1 activity and increased metabolism of 1,12-diamino-3,6,9-triazadodecane to N1-acetyl-1,12-diamino-3,6,9-triazadodecane Homo sapiens
2.3.1.57 metabolism acetylation of triethylenetetramine is increased in SSAT1-overexpressing mice compared with wild-type mice, but SSAT1-deficient mice metabolize TETA at the same rate as the wild-type mice, due to the activity of thialysine acetyltransferase (SSAT2) Mus musculus