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Literature summary extracted from

  • Monshupanee, T.
    Increased bacterial hemolytic activity is conferred by expression of TlyA methyltransferase but not by its 2'-O-methylation of the ribosome (2013), Curr. Microbiol., 67, 61-68.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.226 gene tlyA, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli Mycobacterium tuberculosis
2.1.1.227 gene tlyA, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.226 additional information Mycobacterium tuberculosis the 2'-O-methyltransferase TlyA from Mycobacterium tuberculosis methylates both nucleotide C1409 of 16S rRNA, EC 2.1.1.227, and C1920 of 23S rRNA ?
-
 ?
2.1.1.226 additional information Mycobacterium tuberculosis H37Rv the 2'-O-methyltransferase TlyA from Mycobacterium tuberculosis methylates both nucleotide C1409 of 16S rRNA, EC 2.1.1.227, and C1920 of 23S rRNA ?
-
 ?
2.1.1.226 S-adenosyl-L-methionine + cytidine1920 in 23S rRNA Mycobacterium tuberculosis
-
 S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA
-
 ?
2.1.1.226 S-adenosyl-L-methionine + cytidine1920 in 23S rRNA Mycobacterium tuberculosis H37Rv
-
 S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA
-
 ?
2.1.1.227 additional information Mycobacterium tuberculosis the 2'-O-methyltransferase TlyA from Mycobacterium tuberculosis methylates both nucleotide C1409 of 16S rRNA and C1920 of 23S rRNA, EC 2.1.1.226 ?
-
 ?
2.1.1.227 additional information Mycobacterium tuberculosis H37Rv the 2'-O-methyltransferase TlyA from Mycobacterium tuberculosis methylates both nucleotide C1409 of 16S rRNA and C1920 of 23S rRNA, EC 2.1.1.226 ?
-
 ?
2.1.1.227 S-adenosyl-L-methionine + cytidine1409 in 16S rRNA Mycobacterium tuberculosis
-
 S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA
-
 ?
2.1.1.227 S-adenosyl-L-methionine + cytidine1409 in 16S rRNA Mycobacterium tuberculosis H37Rv
-
 S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.226 Mycobacterium tuberculosis P9WJ63 gene tlyA
-
2.1.1.226 Mycobacterium tuberculosis H37Rv P9WJ63 gene tlyA
-
2.1.1.227 Mycobacterium tuberculosis P9WJ63 gene tlyA
-
2.1.1.227 Mycobacterium tuberculosis H37Rv P9WJ63 gene tlyA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.226 recombinant C-terminally His-tagged enzyme from Escherichia coli Mycobacterium tuberculosis
2.1.1.227 recombinant C-terminally His-tagged enzyme from Escherichia coli Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.226 additional information the 2'-O-methyltransferase TlyA from Mycobacterium tuberculosis methylates both nucleotide C1409 of 16S rRNA, EC 2.1.1.227, and C1920 of 23S rRNA Mycobacterium tuberculosis ?
-
 ?
2.1.1.226 additional information the purified recombinant enzyme TlyA efficiently 2'-O-methylates C1920 in vitro at a molar enzyme to substrate ratio of 1:10 Mycobacterium tuberculosis ?
-
 ?
2.1.1.226 additional information the 2'-O-methyltransferase TlyA from Mycobacterium tuberculosis methylates both nucleotide C1409 of 16S rRNA, EC 2.1.1.227, and C1920 of 23S rRNA Mycobacterium tuberculosis H37Rv ?
-
 ?
2.1.1.226 additional information the purified recombinant enzyme TlyA efficiently 2'-O-methylates C1920 in vitro at a molar enzyme to substrate ratio of 1:10 Mycobacterium tuberculosis H37Rv ?
-
 ?
2.1.1.226 S-adenosyl-L-methionine + cytidine1920 in 23S rRNA
-
 Mycobacterium tuberculosis S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA
-
 ?
2.1.1.226 S-adenosyl-L-methionine + cytidine1920 in 23S rRNA
-
 Mycobacterium tuberculosis H37Rv S-adenosyl-L-homocysteine + 2'-O-methylcytidine1920 in 23S rRNA
-
 ?
2.1.1.227 additional information the 2'-O-methyltransferase TlyA from Mycobacterium tuberculosis methylates both nucleotide C1409 of 16S rRNA and C1920 of 23S rRNA, EC 2.1.1.226 Mycobacterium tuberculosis ?
-
 ?
2.1.1.227 additional information the recombinant enzyme poorly modifies the nucleotide C1409 in vitro in all Escherichia coli ribosomal substrates evaluated: 16S rRNA, 30S ribosomal subunits, and 70S ribosomes Mycobacterium tuberculosis ?
-
 ?
2.1.1.227 additional information the 2'-O-methyltransferase TlyA from Mycobacterium tuberculosis methylates both nucleotide C1409 of 16S rRNA and C1920 of 23S rRNA, EC 2.1.1.226 Mycobacterium tuberculosis H37Rv ?
-
 ?
2.1.1.227 S-adenosyl-L-methionine + cytidine1409 in 16S rRNA
-
 Mycobacterium tuberculosis S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA
-
 ?
2.1.1.227 S-adenosyl-L-methionine + cytidine1409 in 16S rRNA
-
 Mycobacterium tuberculosis H37Rv S-adenosyl-L-homocysteine + 2'-O-methylcytidine1409 in 16S rRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.226 2'-O-methyltransferase
-
 Mycobacterium tuberculosis
2.1.1.226 TlyA
-
 Mycobacterium tuberculosis
2.1.1.227 2'-O-methyltransferase
-
 Mycobacterium tuberculosis
2.1.1.227 TlyA
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.226 S-adenosyl-L-methionine
-
 Mycobacterium tuberculosis
2.1.1.227 S-adenosyl-L-methionine
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.1.1.226 additional information the S4-type RNA recognition domain (residues 5-64), S-adenosyl-methionine binding domain (residues 84-153), SAM-interacting residues (residues 90-94), and four catalytic residues (K69-D154-K182-E238) are conserved in 2'-O-methyltrasferases TlyA Mycobacterium tuberculosis
2.1.1.226 physiological function bacterial 2'-O-methyltransferase TlyA methylates either both nucleotide C1409 of 16S rRNA and C1920 of 23S rRNA. Both ribosomal methylations increase bacterial susceptibility to ribosome targeting antibiotics capreomycin and viomycin. The enzyme also function as a hemolysin, but increased bacterial hemolytic function is not likely a consequence of TlyA-mediated methylations of the ribosome Mycobacterium tuberculosis
2.1.1.227 physiological function bacterial 2'-O-methyltransferase TlyA methylates either both nucleotide C1409 of 16S rRNA and C1920 of 23S rRNA. Both ribosomal methylations increase bacterial susceptibility to ribosome targeting antibiotics capreomycin and viomycin. The enzyme also function as a hemolysin, but increased bacterial hemolytic function is not likely a consequence of TlyA-mediated methylations of the ribosome Mycobacterium tuberculosis