EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.4 | free energy simulations and quantum mechanical/molecular mechanical potential at both 37°C and 85°C. The free energy barriers for glycosylation and deglycosylation are 22.5 and 24.5 kcal/mol at 85°C, respectively. The barrier for deglycosylation decreases with increasing temperature or as a result of the Y61G mutation, consistent with experimental observations. The transition state for glycosylation and deglycosylation is in an oxocarbonium state with the -1 glucose ring having an E3 envelop (or 4H3 half-chair) conformation. A stable moiety exists that may play a role in holding cellulose at the binding site with the correct orientation for the reaction even at 85°C | Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.4 | Thermotoga maritima | Q60032 | - |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.4 | CelA | - |
Thermotoga maritima |